4-phenylpyridine suppresses UVB-induced skin inflammation by targeting c-Src in vitro and in vivo
DC Field | Value | Language |
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dc.contributor.author | Kim, Ju Gyeong | - |
dc.contributor.author | Kang, Ha Yeong | - |
dc.contributor.author | Kim, Min Jeong | - |
dc.contributor.author | Lim, Seokwon | - |
dc.contributor.author | Lee, Chang Joo | - |
dc.contributor.author | Kim, Kyung-Min | - |
dc.contributor.author | Jung, Sung Keun | - |
dc.date.accessioned | 2022-07-19T02:40:29Z | - |
dc.date.available | 2022-07-19T02:40:29Z | - |
dc.date.created | 2022-07-19 | - |
dc.date.issued | 2022-07 | - |
dc.identifier.issn | 1582-1838 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84997 | - |
dc.description.abstract | Acute or repetitive exposure to ultraviolet (UV) cause disruptions to the skin barrier and subsequent inflammatory skin disease. 4-phenylpyridine (4-PP) is a constituent of Brassica campestris L. ssp. Pekinensis and its effect on skin inflammation and molecular target remain unclear. The purpose of this study is to confirm the anti-inflammatory efficacy of 4-PP on UVB-induced skin inflammation in human keratinocytes HaCaT and mouse skin and validation of its molecular target. 4-PP also attenuated UVB-induced phosphorylation of p38/mitogen-activated protein kinase kinase (MKK) 3/6, c-Jun N-terminal kinase 1/2, MKK 4/7, extracellular-signal-regulated kinase 1/2, mitogen-activated protein kinase 1/2. Additionally, 4-PP inhibited UVB-induced phosphorylation of epidermal growth factor receptor (EGFR) Y1068, Y1045 and 854 residues but not the proto-oncogene tyrosine-protein kinase c-Src. Drug affinity responsive target stability assay revealed that 4-PP directly binds to c-Src and inhibits pronase c-proteolysis. Knockdown of c-Src inhibited UVB-induced COX-2 expression and phosphorylation of MAPKs and EGFR in HaCaT cells. Dorsal treatment of 4-PP prevented UVB (0.5 J/cm(2))-induced skin thickness, phosphorylation of EGFR and COX-2 expression in mouse skin. Our findings suggest that 4-PP can be used as anti-inflammatory agent with an effect of skin inflammation by inhibiting the COX-2 expression via suppressing the c-Src/EGFR/MAPKs signalling pathway. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | - |
dc.title | 4-phenylpyridine suppresses UVB-induced skin inflammation by targeting c-Src in vitro and in vivo | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000808446200001 | - |
dc.identifier.doi | 10.1111/jcmm.17422 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, v.26, no.14, pp.3891 - 3901 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85131567308 | - |
dc.citation.endPage | 3901 | - |
dc.citation.startPage | 3891 | - |
dc.citation.title | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | - |
dc.citation.volume | 26 | - |
dc.citation.number | 14 | - |
dc.contributor.affiliatedAuthor | Lim, Seokwon | - |
dc.type.docType | Article; Early Access | - |
dc.subject.keywordAuthor | 4-phenylpyridine | - |
dc.subject.keywordAuthor | Brassica campestris L | - |
dc.subject.keywordAuthor | ssp | - |
dc.subject.keywordAuthor | pekinensis | - |
dc.subject.keywordAuthor | COX-2 | - |
dc.subject.keywordAuthor | c-Src | - |
dc.subject.keywordAuthor | phytochemicals | - |
dc.subject.keywordAuthor | skin inflammation | - |
dc.subject.keywordPlus | COX-2 EXPRESSION | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | RADIATION | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordPlus | KINASE | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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