Clinical evaluation of an innovative isothermal amplification detection system for COVID-19 diagnosis
DC Field | Value | Language |
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dc.contributor.author | Kim, Dami | - |
dc.contributor.author | Kim, Se Jin | - |
dc.contributor.author | Kim, Yu Kyung | - |
dc.contributor.author | Kwon, Ki Tae | - |
dc.contributor.author | Kim, Sanghyo | - |
dc.date.accessioned | 2022-08-24T00:40:09Z | - |
dc.date.available | 2022-08-24T00:40:09Z | - |
dc.date.created | 2022-08-19 | - |
dc.date.issued | 2022-07 | - |
dc.identifier.issn | 1759-9660 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85272 | - |
dc.description.abstract | A pre-integrated system design intended for a point-of-care (POC) and sample-to-result diagnostic platform with nucleic acid amplification has been developed, which is equipment/electricity-free without any permanent instruments or manual sample processing. This semi-integrated system focuses on pandemic situations that are suitable for the Affordable, Sensitive, Specific, User-friendly, Robust and rapid, Equipment-free, and Deliverable to the end-user "ASSURED" concept recommended by the World Health Organization (WHO). Nucleic acid amplification is an essential rate-limiting factor in the performance of integrated systems that involve sample preparation and detection. The ORF1ab (RdRp) gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been targeted by RT-LAMP optimization and evaluation using a commercial hot-pack as a heat source that successfully achieves a femto-scale (<6.8 x 10(2) copies per rxn) limit of detection (LOD) within 40 min (except for the RNA extraction step). Therefore, the prototype system was assessed using COVID-19-suspected clinical samples (eighty eight) and compared with the results of a commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) assay (Allplex SARS-CoV-2 Assay kit (Seegene, Seoul, Republic of Korea)). These innovative approaches achieved over 95% sensitivity and specificity. In conclusion, the developed system using a hot-pack as a heat source is a promising tool that enables the rapid identification of infectious diseases in the real world. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.relation.isPartOf | ANALYTICAL METHODS | - |
dc.title | Clinical evaluation of an innovative isothermal amplification detection system for COVID-19 diagnosis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000815198200001 | - |
dc.identifier.doi | 10.1039/d2ay00815g | - |
dc.identifier.bibliographicCitation | ANALYTICAL METHODS, v.14, no.26, pp.2578 - 2585 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85133137636 | - |
dc.citation.endPage | 2585 | - |
dc.citation.startPage | 2578 | - |
dc.citation.title | ANALYTICAL METHODS | - |
dc.citation.volume | 14 | - |
dc.citation.number | 26 | - |
dc.contributor.affiliatedAuthor | Kim, Sanghyo | - |
dc.type.docType | Article | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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