Dual Beneficial Effects of Methylnissolin-3-O-beta-D-Glucopyranoside on Obesity-Induced Inflammatory Responses in Adipocyte-Macrophage Co-Culture
DC Field | Value | Language |
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dc.contributor.author | Lee, Dahae | - |
dc.contributor.author | Wu, Xiaohua | - |
dc.contributor.author | Lange, Ingo | - |
dc.contributor.author | Cao, Shugeng | - |
dc.contributor.author | Kang, Ki Sung | - |
dc.date.accessioned | 2022-08-31T16:40:06Z | - |
dc.date.available | 2022-08-31T16:40:06Z | - |
dc.date.created | 2022-08-19 | - |
dc.date.issued | 2022-07 | - |
dc.identifier.issn | 2223-7747 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85351 | - |
dc.description.abstract | Methylnissolin-3-O-beta-D-glucopyranoside (MNG) is a pterocarpan analog, which protects EA.hy926 cells against oxidative damage through the Nrf2/HO-1 pathway. However, the effects of MNG on obesity-induced inflammatory responses in adipocyte-macrophage co-culture remain unclear. A differentiated murine preadipocyte cell line (3T3-L1) was co-cultured with a murine macrophage cell line (RAW264.7). Intracellular lipid accumulation was determined using Oil Red O staining. Western blotting was performed to investigate the expression of adipogenesis- and inflammation-associated proteins. Cell culture supernatants were assayed using ELISA kits to measure the levels of proinflammatory cytokines such as interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1). MNG inhibited lipid accumulation and the production of IL-6 and MCP-1 in the 3T3-L1 and RAW264.7 cell co-culture. Moreover, MNG inhibited the protein expression of CCAAT/enhancer-binding protein alpha (C/EBP alpha), C/EBP beta, peroxisome proliferator-activated receptor gamma (PPAR gamma), cyclooxygenase 2 (COX-2), and inducible nitric oxide synthase (iNOS) under the same co-culture conditions. MNG also inhibited IL-6 and MCP-1 production compared with the co-culture control. These findings demonstrate that MNG inhibited lipid accumulation and inflammatory response by downregulating IL-6 and MCP-1 production and protein expression of C/EBP beta, C/EBP alpha, PPAR gamma, COX-2, and iNOS in co-culture conditions with 3T3-L1 and RAW264.7 cells. These results suggest that MNG may be beneficial in preventing obesity-related inflammatory status. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | PLANTS-BASEL | - |
dc.title | Dual Beneficial Effects of Methylnissolin-3-O-beta-D-Glucopyranoside on Obesity-Induced Inflammatory Responses in Adipocyte-Macrophage Co-Culture | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000824326300001 | - |
dc.identifier.doi | 10.3390/plants11131715 | - |
dc.identifier.bibliographicCitation | PLANTS-BASEL, v.11, no.13 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85132858779 | - |
dc.citation.title | PLANTS-BASEL | - |
dc.citation.volume | 11 | - |
dc.citation.number | 13 | - |
dc.contributor.affiliatedAuthor | Lee, Dahae | - |
dc.contributor.affiliatedAuthor | Kang, Ki Sung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | adipocytes | - |
dc.subject.keywordAuthor | macrophage | - |
dc.subject.keywordAuthor | adipogenesis | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordPlus | 6-GINGEROL | - |
dc.subject.keywordPlus | ADIPOGENESIS | - |
dc.subject.keywordPlus | SUPPRESSES | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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