Vinyl-functionalized polyphenolic-carbon dot-based fluorometric turn-off-on biosensor for the dual detection of mercury and cysteine and their in vivo sensing in zebrafish larvae
DC Field | Value | Language |
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dc.contributor.author | Lakshmi, Buddolla Anantha | - |
dc.contributor.author | Sangubotla, Roopkumar | - |
dc.contributor.author | Kim, Jongsung | - |
dc.contributor.author | Kim, Young-Joon | - |
dc.date.accessioned | 2022-10-07T02:40:10Z | - |
dc.date.available | 2022-10-07T02:40:10Z | - |
dc.date.created | 2022-09-22 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 1386-1425 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85630 | - |
dc.description.abstract | The fluorometric turn-off-on biosensor was developed for the ultra-sensitive detection of mercury (Hg2+) and cysteine (Cys) utilizing the highly fluorescent carbon dots (CDs). Herein, the sophisticated low-temperature reflux-mediated reaction was adopted using precursors namely citric acid (CA) and polyphenolic kaempferol (KMP) by using dimethylformamide (DMF) as a solvent. The resulting CDs (i.e., CKCDs) were in the highly negative charged groups (-OH) presented with a bright-orange fluorescence. These CKCDs were functionalized with 4-vinylaniline (4-VA) by employing EDC/NHS coupling reaction, which switched its photoluminescence (PL) towards the strong-blue colored emission and termed as V-CKCDs. The functionalized V-CKCDs can be capable enough to detect mercury via the strong electrostatic interactions between positively charged Hg2+ cations and negatively charged anions (-OH groups). Hence, an adequate fluorescence quenching was observed in V-CKCDs with the lowest concentrations of Hg2+ around 0.5 mu M. Significantly, after adding the complex of V-CKCDs-Hg(2+ )to the Cys, the fluorescence enhancement was observed. This might be attributed from the strong interactions between Hg(2+ )in the fluorescence sensing system and thiol (-SH) moieties from the Cys. The developed V-CKCDs are highly sensitive for detecting Hg(2+ )and Cys, which showed detection limits of 10.6 and 42. 48 nM, respectively. Also, the in vivo studies were investigated in zebrafish larvae using V-CKCDs for the detection of Hg2+ and Cys. The V-CKCDs were investigated in the real water samples and human serum to detect Hg2+ and Cys, respectively. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.relation.isPartOf | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | - |
dc.title | Vinyl-functionalized polyphenolic-carbon dot-based fluorometric turn-off-on biosensor for the dual detection of mercury and cysteine and their in vivo sensing in zebrafish larvae | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000838299300004 | - |
dc.identifier.doi | 10.1016/j.saa.2022.121685 | - |
dc.identifier.bibliographicCitation | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v.282 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85134892606 | - |
dc.citation.title | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | - |
dc.citation.volume | 282 | - |
dc.contributor.affiliatedAuthor | Lakshmi, Buddolla Anantha | - |
dc.contributor.affiliatedAuthor | Sangubotla, Roopkumar | - |
dc.contributor.affiliatedAuthor | Kim, Jongsung | - |
dc.contributor.affiliatedAuthor | Kim, Young-Joon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Fluorometric biosensor | - |
dc.subject.keywordAuthor | Vinyl-functionalized carbon dots | - |
dc.subject.keywordAuthor | Hg2+ | - |
dc.subject.keywordAuthor | Cysteine | - |
dc.subject.keywordAuthor | Turn-off-on | - |
dc.subject.keywordAuthor | Detection Zebrafish | - |
dc.subject.keywordPlus | QUANTUM DOTS | - |
dc.subject.keywordPlus | SELECTIVE DETECTION | - |
dc.subject.keywordPlus | FLUORESCENT-PROBE | - |
dc.subject.keywordPlus | ELECTROCHEMICAL DETECTION | - |
dc.subject.keywordPlus | SILVER NANOPARTICLES | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
dc.subject.keywordPlus | RHODAMINE B | - |
dc.subject.keywordPlus | IONS | - |
dc.subject.keywordPlus | HG2+ | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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