Balanced Free Essential Amino Acids and Resistance Exercise Training Synergistically Improve Dexamethasone-Induced Impairments in Muscle Strength, Endurance, and Insulin Sensitivity in Miceopen access
- Authors
- Jang, Jiwoong; Koh, Jin-Ho; Kim, Yeongmin; Kim, Hee-Joo; Park, Sanghee; Chang, Yewon; Jung, Jiyeon; Wolfe, Robert R.; Kim, Il-Young
- Issue Date
- Sep-2022
- Publisher
- MDPI
- Keywords
- dexamethasone; muscle atrophy; essential amino acids; resistance exercise training; physical performance; mitochondrial biogenesis; neuromuscular junction stability; protein turnover; glucose metabolic flux
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.23, no.17
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 23
- Number
- 17
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85684
- DOI
- 10.3390/ijms23179735
- ISSN
- 1661-6596
- Abstract
- Our previous study shows that an essential amino acid (EAA)-enriched diet attenuates dexamethasone (DEX)-induced declines in muscle mass and strength, as well as insulin sensitivity, but does not affect endurance. In the present study, we hypothesized that the beneficial effects will be synergized by adding resistance exercise training (RET) to EAA, and diet-free EAA would improve endurance. To test hypotheses, mice were randomized into the following four groups: control, EAA, RET, and EAA+RET. All mice except the control were subjected to DEX treatment. We evaluated the cumulative rate of myofibrillar protein synthesis (MPS) using (H2O)-H-2 labeling and mass spectrometry. Neuromuscular junction (NMJ) stability, mitochondrial contents, and molecular signaling were demonstrated in skeletal muscle. Insulin sensitivity and glucose metabolism using C-13(6)-glucose tracing during oral glucose tolerance tests were analyzed. We found that EAA and RET synergistically improve muscle mass and/or strength, and endurance capacity, as well as insulin sensitivity, and glucose metabolism in DEX-treated muscle. These improvements are accomplished, in part, through improvements in myofibrillar protein synthesis, NMJ, fiber type preservation, and/or mitochondrial biogenesis. In conclusion, free EAA supplementation, particularly when combined with RET, can serve as an effective means that counteracts the adverse effects on muscle of DEX that are found frequently in clinical settings.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의예과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.