LC-MS/MS-based Proteomic Analysis of Locally Advanced Rectal Tumors to Identify Biomarkers for Predicting Tumor Response to Neoadjuvant Chemoradiotherapy
DC Field | Value | Language |
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dc.contributor.author | Kim, Kyung-Ok | - |
dc.contributor.author | Duong, Van-An | - |
dc.contributor.author | Han, Na Young | - |
dc.contributor.author | Park, Jong-Moon | - |
dc.contributor.author | Kim, Jung Ho | - |
dc.contributor.author | Lee, Hookeun | - |
dc.contributor.author | Baek, Jeong-Heum | - |
dc.date.accessioned | 2022-10-18T02:40:14Z | - |
dc.date.available | 2022-10-18T02:40:14Z | - |
dc.date.created | 2022-10-01 | - |
dc.date.issued | 2022-09 | - |
dc.identifier.issn | 2233-4203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85781 | - |
dc.description.abstract | Neoadjuvant chemoradiotherapy (nCRT) is a standard therapy used for locally advanced rectal cancer prior to sur- gery, which can more effectively reduce the locoregional recurrence rate and radiation toxicity compared to postoperative chemoradiotherapy. The response of patients to nCRT varies, and thus, robust biomarkers for predicting a pathological complete response are necessary. This study aimed to identify possible biomarkers involved in the complete response/non-response of rec- tal cancer patients to nCRT. Comparative proteomic analysis was performed on rectal tissue samples before and after nCRT. Pro- teins were extracted for label-free proteomic analysis. Western blot and real-time PCR were performed using rectal cancer cell line SNU-503 and radiation-resistant rectal cancer cell line SNU-503R80Gy. A total of 135 up- and 93 down-regulated proteins were identified in the complete response group. Six possible biomarkers were selected to evaluate the expression of proteins and mRNA in SNU-503 and SNU-503R80Gy cell lines. Lyso-phosphatidylcholine acyltransferase 2, annexin A13, aldo-ketose reductase family 1 member B1, and cathelicidin antimicrobial peptide appeared to be potential biomarkers for predicting a pathological complete response to nCRT. This study identified differentially expressed proteins and some potential biomarkers in the complete response group, which would be further validated in future studies. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 사단법인 한국질량분석학회 | - |
dc.relation.isPartOf | Mass Spectrometry Letters | - |
dc.title | LC-MS/MS-based Proteomic Analysis of Locally Advanced Rectal Tumors to Identify Biomarkers for Predicting Tumor Response to Neoadjuvant Chemoradiotherapy | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000889578200003 | - |
dc.identifier.doi | 10.5478/MSL.2022.13.2.84 | - |
dc.identifier.bibliographicCitation | Mass Spectrometry Letters, v.13, no.3, pp.84 - 94 | - |
dc.identifier.kciid | ART002881730 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85139475777 | - |
dc.citation.endPage | 94 | - |
dc.citation.startPage | 84 | - |
dc.citation.title | Mass Spectrometry Letters | - |
dc.citation.volume | 13 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Kim, Kyung-Ok | - |
dc.contributor.affiliatedAuthor | Duong, Van-An | - |
dc.contributor.affiliatedAuthor | Han, Na Young | - |
dc.contributor.affiliatedAuthor | Park, Jong-Moon | - |
dc.contributor.affiliatedAuthor | Kim, Jung Ho | - |
dc.contributor.affiliatedAuthor | Lee, Hookeun | - |
dc.contributor.affiliatedAuthor | Baek, Jeong-Heum | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | LC-MS/MS | - |
dc.subject.keywordAuthor | proteomics | - |
dc.subject.keywordAuthor | neoadjuvant chemoradiotherapy | - |
dc.subject.keywordAuthor | locally advanced rectal cancer | - |
dc.subject.keywordAuthor | biomarker | - |
dc.subject.keywordPlus | SAMPLE PREPARATION METHOD | - |
dc.subject.keywordPlus | POSTOPERATIVE CHEMORADIOTHERAPY | - |
dc.subject.keywordPlus | COLON-CANCER | - |
dc.subject.keywordPlus | CATHELICIDIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | GENE | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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