Caffeic acid phenethyl ester inhibits pseudo-allergic reactions via inhibition of MRGPRX2/MrgprB2-dependent mast cell degranulation
DC Field | Value | Language |
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dc.contributor.author | Adhikari, Nisha | - |
dc.contributor.author | Shim, Won-Sik | - |
dc.date.accessioned | 2022-11-04T03:40:21Z | - |
dc.date.available | 2022-11-04T03:40:21Z | - |
dc.date.created | 2022-11-04 | - |
dc.date.issued | 2022-09 | - |
dc.identifier.issn | 0253-6269 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85965 | - |
dc.description.abstract | Mast cells play essential role in allergic reactionsthrough the process called mast cell degranulation. Recent studies have found that a basic secretagogue compound48/80 (C48/80) induces non-IgE-mediated mastcell degranulation via activation of human Mas-related Gprotein-coupled receptor X2 (MRGPRX2) and mouse MrgprB2. Although previous studies have revealed that caff eicacid (CA) and its derivatives possess anti-allergic eff ects viaIgE-dependent manner, it is largely elusive whether thesecompounds have impact on MRGPRX2/MrgprB2 to exertinhibitory eff ects. Therefore, the present study investigatedwhether CA as well as its derivatives – rosmarinic acid (RA)and caff eic acid phenethyl ester (CAPE) – has the abilityto inhibit the activity of MRGPRX2/MrgprB2 to evokepseudo-allergic eff ects. As a result, it was found that CAPEinhibits C48/80-induced activation of MRGPRX2/MrgprB2,but neither CA nor RA showed discernible inhibition. Furthermore,the β-hexosaminidase release assay showed thatCAPE inhibits mouse peritoneal mast cell degranulationin both IgE-dependent and MrgprB2-dependent manners. Additionally, mouse paw edema induced by C48/80 was dramaticallysuppressed by co-treatment of CAPE, suggestingthat CAPE possesses a protective eff ect on C48/80-evokedpseudo-allergic reactions. The pretreatment of CAPE also signifi cantly decreased scratching bouts of mice evokedby C48/80, demonstrating that CAPE also has an anti-pruriticeff ect. Therefore, these data implicate that CAPE cansuppress pseudo-allergic reactions evoked by C48/80 viaMrgprB2-dependent manner. Finally, molecular dockinganalysis showed that CAPE is predicted to bind to humanMRGPRX2 in the region where C48/80 also binds, implyingthat CAPE can be a competitive inhibitor of MRGPRX2. Inconclusion, it is found that CAPE has the ability to inhibitMRGPRX2/MrgprB2, leading to the prevention of mast celldegranulation and further to the alleviation of mast cell reactions. These results indicate that CAPE as a CA derivativecould be developed as a new protective agent that exerts dualinhibition of mast cell degranulation mediated by IgE andMRGPRX2/MrgprB2. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 대한약학회 | - |
dc.relation.isPartOf | Archives of Pharmacal Research | - |
dc.title | Caffeic acid phenethyl ester inhibits pseudo-allergic reactions via inhibition of MRGPRX2/MrgprB2-dependent mast cell degranulation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000862761100001 | - |
dc.identifier.doi | 10.1007/s12272-022-01405-2 | - |
dc.identifier.bibliographicCitation | Archives of Pharmacal Research, v.45, no.9, pp.644 - 657 | - |
dc.identifier.kciid | ART002892315 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85139120743 | - |
dc.citation.endPage | 657 | - |
dc.citation.startPage | 644 | - |
dc.citation.title | Archives of Pharmacal Research | - |
dc.citation.volume | 45 | - |
dc.citation.number | 9 | - |
dc.contributor.affiliatedAuthor | Adhikari, Nisha | - |
dc.contributor.affiliatedAuthor | Shim, Won-Sik | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Caff eic acid phenethyl ester | - |
dc.subject.keywordAuthor | MRGPRX2 | - |
dc.subject.keywordAuthor | MrgprB2 | - |
dc.subject.keywordAuthor | Mast cell degranulation | - |
dc.subject.keywordAuthor | Allergic reaction | - |
dc.subject.keywordPlus | MURINE MODEL | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CAPE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | MRGPRX2 | - |
dc.subject.keywordPlus | GROWTH | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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