Effects of erythropoietin on osteoblast in the tooth extraction socket in mice periodontitis model
DC Field | Value | Language |
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dc.contributor.author | Bae, Ju-Eun | - |
dc.contributor.author | Hwang, Sung-Min | - |
dc.contributor.author | Aryal, Yam Prasad | - |
dc.contributor.author | Kim, Tae-Young | - |
dc.contributor.author | Sohn, Wern-Joo | - |
dc.contributor.author | An, Seo-Young | - |
dc.contributor.author | Kim, Ji-Youn | - |
dc.contributor.author | An, Chang-Hyeon | - |
dc.contributor.author | Lee, Youngkyun | - |
dc.contributor.author | Kim, Yong-Gun | - |
dc.contributor.author | Park, Jin-Woo | - |
dc.contributor.author | Lee, Jae-Mok | - |
dc.contributor.author | Kim, Jae-Young | - |
dc.contributor.author | Suh, Jo-Young | - |
dc.date.accessioned | 2022-11-11T07:40:32Z | - |
dc.date.available | 2022-11-11T07:40:32Z | - |
dc.date.created | 2022-11-08 | - |
dc.date.issued | 2022-10 | - |
dc.identifier.issn | 1664-042X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86036 | - |
dc.description.abstract | Periodontitis is an excessive inflammatory event in tooth-supporting tissues and can cause tooth loss. We used erythropoietin (EPO), which has been reported to play an important role in bone healing and modulation of angiogenesis, as a therapeutic agent in vivo and in vitro experimental models to analyze its effect on periodontitis. First, EPO was applied to in vitro MC3T3-E1 cells and human periodontal ligament fibroblast (hPDLF) cells to examine its function in altered cellular events and gene expression patterns. In vitro cultivation of MC3T3-E1 and hPDLF cells with 10 IU/ml EPO at 24 and 48 h showed an obvious increase in cell proliferation. Interestingly, EPO treatment altered the expression of osteogenesis-related molecules, including alkaline phosphatase (ALP), bone morphogenetic protein-2 (BMP-2), and osteocalcin (OC) in MC3T3-E1 cells but not in hPDLF cells. In particular, MC3T3-E1 cells showed increased expression of ALP, BMP-2, and OC on day 5, while hPDLF cells showed increased expression of BMP-2, and OC on day 14. Based on the in vitro examination, we evaluated the effect of EPO on bone formation using an experimentally-induced animal periodontitis model. After the induction of periodontitis in the maxillary left second M, 10 IU/ml of EPO was locally applied to the extraction tooth sockets. Histomorphological examination using Masson's trichrome (MTC) staining showed facilitated bone formation in the EPO-treated groups after 14 days. Similarly, stronger positive reactions against vascular endothelial growth factor (VEGF), cluster of differentiation 31 (CD31), runt-related transcription factor 2 (RUNX2), and osteocalcin (OC) were detected in the EPO-treated group compared to the control. Meanwhile, myeloperoxidase, an inflammatory marker, was decreased in the EPO-treated group on days 1 and 5. Overall, EPO facilitates bone healing and regeneration through altered signaling regulation and modulation of inflammation in the osteoblast cell lineage and to a lesser extent in hPDLF cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.relation.isPartOf | FRONTIERS IN PHYSIOLOGY | - |
dc.title | Effects of erythropoietin on osteoblast in the tooth extraction socket in mice periodontitis model | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000874042300001 | - |
dc.identifier.doi | 10.3389/fphys.2022.987625 | - |
dc.identifier.bibliographicCitation | FRONTIERS IN PHYSIOLOGY, v.13 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85140391312 | - |
dc.citation.title | FRONTIERS IN PHYSIOLOGY | - |
dc.citation.volume | 13 | - |
dc.contributor.affiliatedAuthor | Kim, Ji-Youn | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | human periodontal ligament fibroblast cell | - |
dc.subject.keywordAuthor | local delivery | - |
dc.subject.keywordAuthor | MC3T3-E1 cells | - |
dc.subject.keywordAuthor | osteoblast | - |
dc.subject.keywordAuthor | periodontitis | - |
dc.subject.keywordAuthor | tooth loss | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | BONE | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.subject.keywordPlus | SITES | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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