Development and validation of a highly sensitive LC-MS/MS method for the determination of acacetin in human plasma and its application to a protein binding study
DC Field | Value | Language |
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dc.contributor.author | Kim, Sang-Bum | - |
dc.contributor.author | Lee, Taehun | - |
dc.contributor.author | Lee, Hun Seok | - |
dc.contributor.author | Song, Chung Kil | - |
dc.contributor.author | Cho, Hyun-Jong | - |
dc.contributor.author | Kim, Dae-Duk | - |
dc.contributor.author | Maeng, Han-Joo | - |
dc.contributor.author | Yoon, In-Soo | - |
dc.date.available | 2020-02-28T02:45:56Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.issn | 0253-6269 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8614 | - |
dc.description.abstract | A highly sensitive bioanalytical method for the quantification of acacetin in human plasma was developed and comprehensively validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A minimal volume of human plasma sample (20 mu L) was prepared by simple deproteinization with 80 mu L of acetonitrile. Chromatographic separation was performed using Kinetex C-18 column with an isocratic mobile phase consisting of water and acetonitrile (20:80, v/v) containing 0.1 % formic acid at a flow rate of 0.3 mL/min over a total run time of 2.0 min. Mass spectrometric detection was performed using multiple reaction-monitoring modes at the mass/charge transitions m/z 285.22 -> 242.17 for acacetin and m/z 277.59 -> 175.04 for chlorpropamide (internal standard). The calibration curve was linear over the range of 0.1-500 ng/mL with a lower limit of quantitation of 0.1 ng/mL. The coefficients of variation for both intra- and inter-day validation were less than 11.9 %, and the intra- and inter-day accuracy ranged from 96.8 to 108 %. Mean recovery of acacetin in human plasma was within the range of 91.5-95.6 %. This validated LC-MS/MS method was successfully applied to a human plasma protein binding study that indicated extensive and concentration-independent protein binding of acacetin in human plasma. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PHARMACEUTICAL SOC KOREA | - |
dc.relation.isPartOf | ARCHIVES OF PHARMACAL RESEARCH | - |
dc.title | Development and validation of a highly sensitive LC-MS/MS method for the determination of acacetin in human plasma and its application to a protein binding study | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000370263600008 | - |
dc.identifier.doi | 10.1007/s12272-015-0697-1 | - |
dc.identifier.bibliographicCitation | ARCHIVES OF PHARMACAL RESEARCH, v.39, no.2, pp.213 - 220 | - |
dc.identifier.kciid | ART002079542 | - |
dc.identifier.scopusid | 2-s2.0-84959453272 | - |
dc.citation.endPage | 220 | - |
dc.citation.startPage | 213 | - |
dc.citation.title | ARCHIVES OF PHARMACAL RESEARCH | - |
dc.citation.volume | 39 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Maeng, Han-Joo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Acacetin | - |
dc.subject.keywordAuthor | LC-MS/MS | - |
dc.subject.keywordAuthor | Human plasma | - |
dc.subject.keywordAuthor | Protein binding | - |
dc.subject.keywordPlus | TANDEM MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | EQUILIBRIUM DIALYSIS | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | BIOAVAILABILITY | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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