Diagnosis of tuberculosis using a liquid crystal-based optical sensor
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyeong Jin | - |
dc.contributor.author | Rim, Jinseob | - |
dc.contributor.author | Jang, Chang-Hyun | - |
dc.date.available | 2020-02-28T03:41:15Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.issn | 1598-5032 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8642 | - |
dc.description.abstract | In this study, we developed an advanced liquid crystal (LC)-based biosensor using polymeric surfaces to detect anti-tuberculosis antibodies, specifically in solution. To manufacture the anisotropic nanostructures, poly (dimethylsiloxane) was stretched and subsequently relieved. Before the deposition of a gold film, the nanostructures were replicated on poly(urethane acrylate)-coated silica substrates. Then, tuberculous antigens (ESAT-6) were immobilized onto the surfaces previously treated via N-hydroxysuccinimide (NHS)/N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) chemistry. The LCs (4-cyano-4'-pentylbiphenyl, 5CB), which aligned in parallel with the plane of the antigen-immobilized surface, showed a uniform appearance. However, after incubation of the anti-tuberculosis antibody (anti-ESAT-6) on the antigen-immobilized surface, 5CB showed a random appearance that corresponded to its random orientation. In control experiments, the tuberculous antigen-immobilized surfaces retained a uniform appearance after incubation with another anti-tuberculosis antibody, human serum albumin, and phosphate-buffered saline. These results verified that the tuberculous antigen-antibody complexes on the surface masked the anisotropic surface topography, which led to the transition of 5CB from a uniform orientation to a random one. Consequently, LC sensing systems based on nanostructured polymeric surfaces could be used in the labelfree detection of anti-tuberculosis antibodies, and they could be expanded as a feasible application in the immunoassay of tuberculosis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.relation.isPartOf | MACROMOLECULAR RESEARCH | - |
dc.subject | PULMONARY TUBERCULOSIS | - |
dc.subject | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject | AMPLIFICATION | - |
dc.subject | SURFACES | - |
dc.subject | ESAT-6 | - |
dc.subject | CFP-10 | - |
dc.subject | TIME | - |
dc.title | Diagnosis of tuberculosis using a liquid crystal-based optical sensor | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000371255900005 | - |
dc.identifier.doi | 10.1007/s13233-016-4019-3 | - |
dc.identifier.bibliographicCitation | MACROMOLECULAR RESEARCH, v.24, no.2, pp.123 - 130 | - |
dc.identifier.kciid | ART002080431 | - |
dc.identifier.scopusid | 2-s2.0-84961285995 | - |
dc.citation.endPage | 130 | - |
dc.citation.startPage | 123 | - |
dc.citation.title | MACROMOLECULAR RESEARCH | - |
dc.citation.volume | 24 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Kim, Hyeong Jin | - |
dc.contributor.affiliatedAuthor | Rim, Jinseob | - |
dc.contributor.affiliatedAuthor | Jang, Chang-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | liquid crystal | - |
dc.subject.keywordAuthor | 4-cyano-4 &apos | - |
dc.subject.keywordAuthor | -pentylbiphenyl (5CB) | - |
dc.subject.keywordAuthor | polymeric substrate | - |
dc.subject.keywordAuthor | antigen-antibody complex | - |
dc.subject.keywordAuthor | tuberculosis | - |
dc.subject.keywordPlus | PULMONARY TUBERCULOSIS | - |
dc.subject.keywordPlus | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | ESAT-6 | - |
dc.subject.keywordPlus | CFP-10 | - |
dc.subject.keywordPlus | TIME | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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