Estrogenic Activity of 4-Hydroxy-Benzoic Acid from Acer tegmentosum via Estrogen Receptor alpha-Dependent Signaling Pathways
DC Field | Value | Language |
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dc.contributor.author | Nguyen, Quynh Nhu | - |
dc.contributor.author | Lee, Seoung Rak | - |
dc.contributor.author | Kim, Baolo | - |
dc.contributor.author | Hong, Joo-Hyun | - |
dc.contributor.author | Jang, Yoon Seo | - |
dc.contributor.author | Lee, Da Eun | - |
dc.contributor.author | Pang, Changhyun | - |
dc.contributor.author | Kang, Ki Sung | - |
dc.contributor.author | Kim, Ki Hyun | - |
dc.date.accessioned | 2023-01-19T01:41:46Z | - |
dc.date.available | 2023-01-19T01:41:46Z | - |
dc.date.created | 2023-01-18 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 2223-7747 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86684 | - |
dc.description.abstract | Acer tegmentosum, a deciduous tree belonging to Aceraceae, has been used in traditional oriental medicine for treating hepatic disorders, such as hepatitis, cirrhosis, and liver cancer. We evaluated the estrogen-like effects of A. tegmentosum using an estrogen receptor (ER)-positive breast cancer cell line, namely MCF-7, to identify potential phytoestrogens and found that an aqueous extract of A. tegmentosum promoted cell proliferation in MCF-7 cells. Five phenolic compounds (1-5) were separated and identified from the active fraction using bioassay-guided fractionation of crude A. tegmentosum extract and phytochemical analysis. The chemical structures of the compounds were characterized as vanillic acid (1), 4-hydroxy-benzoic acid (2), syringic acid (3), isoscopoletin (4), and (E)-ferulic acid (5) based on the analysis of their nuclear magnetic resonance spectra and liquid chromatography-mass spectrometry data. All five compounds were evaluated using an E-screen assay for their estrogen-like effects on MCF-7 cells. Among the tested compounds, only 4-hydroxy-benzoic acid (2) promoted the proliferation of MCF-7 cells, which was mitigated by the ER antagonist, ICI 182,780. The mechanism underlying the estrogen-like effect of 4-hydroxy-benzoic acid (2) was evaluated via western blotting analysis to determine the expression levels of extracellular signal-regulated kinase (ERK), phosphoinositide 3-kinase (PI3K), serine/threonine kinase (AKT), and ER alpha. Our results demonstrated that 4-hydroxy-benzoic acid (2) induced the increase in the protein expression levels of p-ERK, p-AKT, p-PI3K, and p-Er alpha, concentration dependently. Collectively, these experimental results suggest that 4-hydroxy-benzoic acid (2) is responsible for the estrogen-like effects of A. tegmentosum and may potentially aid in the control of estrogenic effects during menopause. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | PLANTS-BASEL | - |
dc.title | Estrogenic Activity of 4-Hydroxy-Benzoic Acid from Acer tegmentosum via Estrogen Receptor alpha-Dependent Signaling Pathways | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000897329900001 | - |
dc.identifier.doi | 10.3390/plants11233387 | - |
dc.identifier.bibliographicCitation | PLANTS-BASEL, v.11, no.23 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85143508523 | - |
dc.citation.title | PLANTS-BASEL | - |
dc.citation.volume | 11 | - |
dc.citation.number | 23 | - |
dc.contributor.affiliatedAuthor | Nguyen, Quynh Nhu | - |
dc.contributor.affiliatedAuthor | Kim, Baolo | - |
dc.contributor.affiliatedAuthor | Kang, Ki Sung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Acer tegmentosum | - |
dc.subject.keywordAuthor | 4-hydroxy-benzoic acid | - |
dc.subject.keywordAuthor | phytoestrogens | - |
dc.subject.keywordAuthor | estrogen receptor | - |
dc.subject.keywordPlus | P-HYDROXYBENZOIC ACID | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | PHYTOESTROGENS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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