Hepatic DGAT2 gene expression is regulated by the synergistic action of ChREBP and SP1 in HepG2 cells
DC Field | Value | Language |
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dc.contributor.author | Shin, Eunji | - |
dc.contributor.author | Bae, Jin-Sik | - |
dc.contributor.author | Han, Jung-Youn | - |
dc.contributor.author | Lee, Junghoon | - |
dc.contributor.author | Jeong, Yun-Seung | - |
dc.contributor.author | Lee, Ho-Jae | - |
dc.contributor.author | Ahn, Yong-Ho | - |
dc.contributor.author | Cha, Ji-Young | - |
dc.date.available | 2020-02-28T03:41:38Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2016-01-02 | - |
dc.identifier.issn | 1976-8354 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8668 | - |
dc.description.abstract | Diacylglycerol acyltransferase 2 (DGAT2) catalyzes the final step of triglyceride synthesis and plays a critical role in the development of fatty liver disease and insulin resistance. The expression of DGAT2 is mostly induced by dietary carbohydrate in the mammalian liver; however, the transcription factors that regulate DGAT2 expression have yet to be identified. In this study, we investigated the molecular mechanism underlying the glucose-induced transcriptional regulation of human DGAT2 in HepG2 cells. Human DGAT2 expression was increased by glucose in HepG2 cells. Transfection studies of the DGAT2 promoter-luciferase reporter construct in vitro and in silico analysis identified two glucose-responsive regions in the DGAT2 promoter. Each region contains one ChREBP/MLX (carbohydrate response element binding protein/Max-like protein) binding site (ChoRE, -539 to -551 and -6067 to -6083) and one specificity protein 1 (SP1) binding site (GC-rich motif, -556 to -572 and -6016 to -6032). Mutational analysis showed that both ChREBP/MLX and SP1 sites are required for the glucose-induced transcription of DGAT2. Gel shift assays and chromatin immunoprecipitation assays demonstrated that ChREBP and SP1 bind directly to ChoRE and the GC-rich motif, respectively. High glucose promoted the recruitment of ChREBP to ChoRE, whereas SP1 was recruited to the GC-rich motif even under low-glucose conditions. These data demonstrate that both ChREBP and SP1 are key factors to regulate the expression of human DGAT2 by glucose. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.relation.isPartOf | ANIMAL CELLS AND SYSTEMS | - |
dc.subject | ELEMENT-BINDING PROTEIN | - |
dc.subject | DIACYLGLYCEROL ACYLTRANSFERASE | - |
dc.subject | GLUCOSE-METABOLISM | - |
dc.subject | INSULIN-RESISTANCE | - |
dc.subject | CARBOHYDRATE | - |
dc.subject | STEATOSIS | - |
dc.subject | ENZYME | - |
dc.subject | MICE | - |
dc.title | Hepatic DGAT2 gene expression is regulated by the synergistic action of ChREBP and SP1 in HepG2 cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000371742900002 | - |
dc.identifier.doi | 10.1080/19768354.2015.1131738 | - |
dc.identifier.bibliographicCitation | ANIMAL CELLS AND SYSTEMS, v.20, no.1, pp.7 - 14 | - |
dc.identifier.kciid | ART002080220 | - |
dc.identifier.scopusid | 2-s2.0-84959161068 | - |
dc.citation.endPage | 14 | - |
dc.citation.startPage | 7 | - |
dc.citation.title | ANIMAL CELLS AND SYSTEMS | - |
dc.citation.volume | 20 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Shin, Eunji | - |
dc.contributor.affiliatedAuthor | Bae, Jin-Sik | - |
dc.contributor.affiliatedAuthor | Han, Jung-Youn | - |
dc.contributor.affiliatedAuthor | Lee, Junghoon | - |
dc.contributor.affiliatedAuthor | Jeong, Yun-Seung | - |
dc.contributor.affiliatedAuthor | Lee, Ho-Jae | - |
dc.contributor.affiliatedAuthor | Ahn, Yong-Ho | - |
dc.contributor.affiliatedAuthor | Cha, Ji-Young | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | DGAT2 | - |
dc.subject.keywordAuthor | ChREBP | - |
dc.subject.keywordAuthor | SP1 | - |
dc.subject.keywordAuthor | triglyceride synthesis | - |
dc.subject.keywordAuthor | transcriptional regulation | - |
dc.subject.keywordPlus | ELEMENT-BINDING PROTEIN | - |
dc.subject.keywordPlus | DIACYLGLYCEROL ACYLTRANSFERASE | - |
dc.subject.keywordPlus | GLUCOSE-METABOLISM | - |
dc.subject.keywordPlus | INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | CARBOHYDRATE | - |
dc.subject.keywordPlus | STEATOSIS | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | MICE | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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