The new insight into the inflammatory response following focused ultrasound-mediated blood-brain barrier disruption
DC Field | Value | Language |
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dc.contributor.author | Choi, Hyo Jin | - |
dc.contributor.author | Han, Mun | - |
dc.contributor.author | Seo, Hyeon | - |
dc.contributor.author | Park, Chan Yuk | - |
dc.contributor.author | Lee, Eun-Hee | - |
dc.contributor.author | Park, Juyoung | - |
dc.date.accessioned | 2023-01-19T01:42:05Z | - |
dc.date.available | 2023-01-19T01:42:05Z | - |
dc.date.created | 2023-01-18 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 2045-8118 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86706 | - |
dc.description.abstract | Background: Despite the great potential of FUS-BBB disruption (FUS-BBBD), it is still controversial whether FUS-BBBD acts as an inducing factor of neuro-inflammation or not, and the biological responses after FUS-BBBD triggers the inflammatory process are poorly understood. The aim of this study is to investigate the safety window for FUS levels based on a comprehensive safety assessment.Methods: The mice were treated with two different ultrasound parameters (0.25 MPa and 0.42 MPa) in the thalamus region of brain. The efficacy of BBB opening was verified by dynamic contrast-enhanced MRI (DCE-MRI) and the cavitation monitoring. The transcriptome analysis was performed to investigate the molecular response for the two BBBD conditions after FUS-mediated BBB opening in time-dependent manners. Histological analysis was used for evaluation of the tissue damage, neuronal degeneration, and activation of glial cells induced by FUS-BBBD.Results: The BBBD, as quantified by the Ktrans, was approximately threefold higher in 0.42 MPa-treated group than 0.25 MPa-treated group. While the minimal tissue/cellular damage was found in 0.25 MPa-treated group, visible damages containing microhemorrhages and degenerating neurons were detected in 0.42 MPa-treated group in accordance with the extent of BBBD. In transcriptome analysis, 0.42 MPa-treated group exhibited highly dynamic changes in the expression levels of an inflammatory response or NF-kappa B pathway-relative genes in a time-dependent manner whereas, 0.25 MPa was not altered. Interestingly, although it is clear that 0.42 MPa induces neuroinflammation through glial activation, neuroprotective properties were evident by the expression of A2-type astrocytes.Conclusions: Our findings propose that a well-defined BBBD parameter of 0.25 MPa could ensure the safety without cellular/tissue damage or sterile inflammatory response in the brain. Furthermore, the fact that the excessive sonication parameters at 0.42 MPa could induce a sterile inflammation response via glial activation suggested the possibility that could lead to tissue repair toward the homeostasis of the brain microenvironment through A2-type reactive astrocytes. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.relation.isPartOf | FLUIDS AND BARRIERS OF THE CNS | - |
dc.title | The new insight into the inflammatory response following focused ultrasound-mediated blood-brain barrier disruption | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000903526200001 | - |
dc.identifier.doi | 10.1186/s12987-022-00402-3 | - |
dc.identifier.bibliographicCitation | FLUIDS AND BARRIERS OF THE CNS, v.19, no.1 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85144636854 | - |
dc.citation.title | FLUIDS AND BARRIERS OF THE CNS | - |
dc.citation.volume | 19 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Park, Juyoung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Blood-brain barrier | - |
dc.subject.keywordAuthor | Focused ultrasound | - |
dc.subject.keywordAuthor | Acute neuroinflammation | - |
dc.subject.keywordAuthor | Genome profiling | - |
dc.subject.keywordAuthor | Astrocyte | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | CONTRAST AGENT | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | MICROBUBBLES | - |
dc.subject.keywordPlus | PERMEABILITY | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | DOXORUBICIN | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PRESSURES | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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