Anti-neuroinflammatory effects of alkaloid-enriched extract from Huperzia serrata on lipopolysaccharide-stimulated BV-2 microglial cells
DC Field | Value | Language |
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dc.contributor.author | Dang, Thu Kim | - |
dc.contributor.author | Hong, Seong-Min | - |
dc.contributor.author | Dao, Vui Thi | - |
dc.contributor.author | Tran, Phuong Thi Thu | - |
dc.contributor.author | Tran, Hiep Tuan | - |
dc.contributor.author | Do, Giang Hoang | - |
dc.contributor.author | Hai, Thanh Nguyen | - |
dc.contributor.author | Pham, Hang Thi Nguyet | - |
dc.contributor.author | Kim, Sun Yeou | - |
dc.date.accessioned | 2023-01-23T01:40:05Z | - |
dc.date.available | 2023-01-23T01:40:05Z | - |
dc.date.created | 2023-01-23 | - |
dc.date.issued | 2023-12 | - |
dc.identifier.issn | 1388-0209 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86758 | - |
dc.description.abstract | Context Alkaloid-enriched extract of Huperzia serrata (Thunb.) Trevis (Lycopodiaceae) (HsAE) can potentially be used to manage neuronal disorders. Objective This study determines the anti-neuroinflammatory effects of HsAE on lipopolysaccharide (LPS)-stimulated BV-2 microglial cells and the underlying mechanisms. Materials and methods BV-2 cells were pre- or post-treated with different concentrations of HsAE (25-150 mu g/mL) for 30 min before or after LPS induction. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and no cytotoxicity was found. Nitric oxide (NO) concentration was determined using Griess reagent. The levels of prostaglandin E2 (PGE2), tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and IL-6 were determined using enzyme-linked immunosorbent assay. The levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 and the phosphorylation of mitogen-activated protein kinase (MAPK) were analyzed using western blotting. Results HsAE reduced LPS-induced NO production with half-maximal inhibitory concentration values of 99.79 and 92.40 mu g/mL at pre- and post-treatment, respectively. Pre-treatment with HsAE at concentrations of 50, 100, and 150 mu g/mL completely inhibited the secretion of PGE2, TNF-alpha, IL-6, and IL-1 beta compared to post-treatment with HsAE. This suggests that prophylactic treatment is better than post-inflammation treatment. HsAE decreased the expression levels of iNOS and COX-2 and attenuated the secretion of pro-inflammatory factors by downregulating the phosphorylation of p38 and extracellular signal-regulated protein kinase in the MAPK signaling pathway. Discussion and Conclusions HsAE exerts anti-neuroinflammatory effects on LPS-stimulated BV-2 cells, suggesting that it may be a potential candidate for the treatment of neuroinflammation in neurodegenerative diseases. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.relation.isPartOf | PHARMACEUTICAL BIOLOGY | - |
dc.title | Anti-neuroinflammatory effects of alkaloid-enriched extract from Huperzia serrata on lipopolysaccharide-stimulated BV-2 microglial cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000910698900001 | - |
dc.identifier.doi | 10.1080/13880209.2022.2159450 | - |
dc.identifier.bibliographicCitation | PHARMACEUTICAL BIOLOGY, v.61, no.1, pp.135 - 143 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85145980061 | - |
dc.citation.endPage | 143 | - |
dc.citation.startPage | 135 | - |
dc.citation.title | PHARMACEUTICAL BIOLOGY | - |
dc.citation.volume | 61 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Hong, Seong-Min | - |
dc.contributor.affiliatedAuthor | Kim, Sun Yeou | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Huperzine A | - |
dc.subject.keywordAuthor | neurodegenerative diseases | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Medical Laboratory Technology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Medical Laboratory Technology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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