miRNA-6515-5p regulates particulate matter-induced inflammatory responses by targeting CSF3 in human bronchial epithelial cells
DC Field | Value | Language |
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dc.contributor.author | Son, Eun Suk | - |
dc.contributor.author | Ko, Ui Won | - |
dc.contributor.author | Jeong, Hee-Yeon | - |
dc.contributor.author | Park, So Young | - |
dc.contributor.author | Lee, Young Eun | - |
dc.contributor.author | Park, Jeong-Woong | - |
dc.contributor.author | Jeong, Sung Hwan | - |
dc.contributor.author | Kim, Se-Hee | - |
dc.contributor.author | Kyung, Sun Young | - |
dc.date.accessioned | 2023-02-17T10:40:15Z | - |
dc.date.available | 2023-02-17T10:40:15Z | - |
dc.date.created | 2023-01-04 | - |
dc.date.issued | 2022-10 | - |
dc.identifier.issn | 0887-2333 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86888 | - |
dc.description.abstract | Particulate matter (PM) is associated with the incidence, exacerbation, and mortality of variable respiratory diseases. However, the molecular mechanisms of PM10-mediated inflammation are unclear. We identified microRNAs (miRNAs) and messenger RNAs (mRNAs) related to the inflammatory response in PM10-exposed bronchial epithelial cells using next-generation sequencing. Of the miRNAs, miR-6515-5p was significantly downregulated in PM10-exposed human bronchial epithelial BEAS-2B cells. miR-6515-5p regulated the production of pro-inflammatory cytokines (IL-6 and IL-8) and the expression of inflammatory genes (IL-1β, IL-6, IL-8, TNF-α, CXCL-1, and MCP-1) via MAPK/ERK signaling; overexpression of miR-6515-5p using a mimic inhibited PM10-induced inflammatory responses via inactivation of the ERK pathway, whereas downregulation of miR-6515-5p via an inhibitor significantly increased inflammation in PM10-exposed cells via activation of ERK. Furthermore, we identified colony stimulating factor 3 (CSF3) as a target gene of miR-6515-5p using TargetScanHuman, and confirmed the association between miR-6515-5p and CSF3 using a luciferase reporter assay. Furthermore, we found that mRNA and protein levels of CSF3 were negatively regulated by miR-6515-5p. Inhibition of CSF3 by small interfering RNA significantly reduced the expression and production of inflammatory markers in PM10-exposed cells by inactivating the MAPK/ERK signaling pathway. Therefore, we suggest that miR-6515-5p regulates PM10-induced inflammatory responses by targeting CSF3 via MAPK/ERK signaling in bronchial epithelial cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.relation.isPartOf | Toxicology in Vitro | - |
dc.title | miRNA-6515-5p regulates particulate matter-induced inflammatory responses by targeting CSF3 in human bronchial epithelial cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000913784400003 | - |
dc.identifier.doi | 10.1016/j.tiv.2022.105428 | - |
dc.identifier.bibliographicCitation | Toxicology in Vitro, v.84 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85134573602 | - |
dc.citation.title | Toxicology in Vitro | - |
dc.citation.volume | 84 | - |
dc.contributor.affiliatedAuthor | Son, Eun Suk | - |
dc.contributor.affiliatedAuthor | Ko, Ui Won | - |
dc.contributor.affiliatedAuthor | Jeong, Hee-Yeon | - |
dc.contributor.affiliatedAuthor | Park, So Young | - |
dc.contributor.affiliatedAuthor | Lee, Young Eun | - |
dc.contributor.affiliatedAuthor | Park, Jeong-Woong | - |
dc.contributor.affiliatedAuthor | Jeong, Sung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Se-Hee | - |
dc.contributor.affiliatedAuthor | Kyung, Sun Young | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | CSF3 | - |
dc.subject.keywordAuthor | ERK | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | microRNA | - |
dc.subject.keywordAuthor | Particulate matter | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | EXPOSURE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | MICRORNAS | - |
dc.subject.keywordPlus | IL-8 | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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