A novel paper-based lysis strip for SARS-CoV-2 RNA detection at low resource settings
DC Field | Value | Language |
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dc.contributor.author | Lee, Songhyun | - |
dc.contributor.author | Kim, SeJin | - |
dc.contributor.author | Kim, Sanghyo | - |
dc.date.accessioned | 2023-03-14T06:40:11Z | - |
dc.date.available | 2023-03-14T06:40:11Z | - |
dc.date.created | 2023-03-14 | - |
dc.date.issued | 2023-03 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/87066 | - |
dc.description.abstract | Infectious respiratory diseases such as COVID-19 are serious and global concerns from the past to the present. To isolate the spread of infectious diseases even in the absence of a health system, a simple, inexpensive, reliable, sensitive, and selective molecular diagnosis platform for Point of Care Test (POCT) is required. Especially, the nucleic acid extraction step is difficult to perform out of laboratory. Here, we propose a paper-based lysis (PBL) strip for nucleic acid extraction, especially in low-resource settings (LRS). PBL strips are suitable for isolating RNA from viruses with biological interference and inhibitors. We optimized the buffer compositions and membranes of the strip. A simple preparation method using a PBL strip could obtain an eluent for downstream inspection within 20 min. Overall, 104 copies/swaps were detected for 20 min for amplification in combination with Reverse Transcription Loop-Mediated Amplification (RT-LAMP). | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | ANALYTICAL BIOCHEMISTRY | - |
dc.title | A novel paper-based lysis strip for SARS-CoV-2 RNA detection at low resource settings | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000925221800001 | - |
dc.identifier.doi | 10.1016/j.ab.2023.115037 | - |
dc.identifier.bibliographicCitation | ANALYTICAL BIOCHEMISTRY, v.664 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85146050439 | - |
dc.citation.title | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.volume | 664 | - |
dc.contributor.affiliatedAuthor | Lee, Songhyun | - |
dc.contributor.affiliatedAuthor | Kim, SeJin | - |
dc.contributor.affiliatedAuthor | Kim, Sanghyo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | ARS-CoV-2 | - |
dc.subject.keywordAuthor | RNA extraction | - |
dc.subject.keywordAuthor | Lysis | - |
dc.subject.keywordAuthor | Point of care test | - |
dc.subject.keywordAuthor | Paper-based | - |
dc.subject.keywordPlus | POINT-OF-CARE | - |
dc.subject.keywordPlus | SAMPLE PREPARATION | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | NITROCELLULOSE | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | DEVICE | - |
dc.subject.keywordPlus | CELLS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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