Reducing tumor invasiveness by ramucirumab and TGF-beta receptor kinase inhibitor in a diffuse-type gastric cancer patient-derived cell model
DC Field | Value | Language |
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dc.contributor.author | Lee, Song-Yi | - |
dc.contributor.author | Byeon, Seonggyu | - |
dc.contributor.author | Ko, Jihoon | - |
dc.contributor.author | Hyung, Sujin | - |
dc.contributor.author | Lee, In-Kyoung | - |
dc.contributor.author | Li Jeon, Noo | - |
dc.contributor.author | Hong, Jung Yong | - |
dc.contributor.author | Kim, Seung Tae | - |
dc.contributor.author | Park, Se Hoon | - |
dc.contributor.author | Lee, Jeeyun | - |
dc.date.accessioned | 2023-03-27T06:43:09Z | - |
dc.date.available | 2023-03-27T06:43:09Z | - |
dc.date.created | 2023-03-27 | - |
dc.date.issued | 2021-10 | - |
dc.identifier.issn | 2045-7634 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/87312 | - |
dc.description.abstract | Background Diffuse-type gastric cancer (GC) is known to be more aggressive and relatively resistant to conventional chemotherapy. Hence, more optimized treatment strategy is urgently needed in diffuse-type GC. Methods Using a panel of 10 GC cell lines and 3 GC patient-derived cells (PDCs), we identified cell lines with high EMTness which is a distinct feature for diffuse-type GC. We treated GC cells with high EMTness with ramucirumab alone, TGF-beta receptor kinase inhibitor (TEW-7197) alone, or in combination to investigate the drug's effects on invasiveness, spheroid formation, EMT marker expression, and tumor-induced angiogenesis using a spheroid-on-a-chip model. Results Both TEW-7197 and ramucirumab treatments profoundly decreased invasiveness of EMT-high cell lines and PDCs. With a 3D tumor spheroid-on-a-chip, we identified versatile influence of co-treatment on cancer cell-induced blood vessel formation as well as on EMT progression in tumor spheroids. The 3D tumor spheroid-on-a-chip demonstrated that TEW-7197 + ramucirumab combination significantly decreased PDC-induced vessel formation. Conclusions In this study, we showed TEW-7197 and ramucirumab considerably decreased invasiveness, thus EMTness in a panel of diffuse-type GC cell lines including GC PDCs. Taken together, we confirmed that combination of TEW-7197 and ramucirumab reduced tumor spheroid and GC PDC-induced blood vessel formation concomitantly in the spheroid-on-a-chip model. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | CANCER MEDICINE | - |
dc.title | Reducing tumor invasiveness by ramucirumab and TGF-beta receptor kinase inhibitor in a diffuse-type gastric cancer patient-derived cell model | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000697896900001 | - |
dc.identifier.doi | 10.1002/cam4.4259 | - |
dc.identifier.bibliographicCitation | CANCER MEDICINE, v.10, no.20, pp.7253 - 7262 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85115053787 | - |
dc.citation.endPage | 7262 | - |
dc.citation.startPage | 7253 | - |
dc.citation.title | CANCER MEDICINE | - |
dc.citation.volume | 10 | - |
dc.citation.number | 20 | - |
dc.contributor.affiliatedAuthor | Ko, Jihoon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | angiogenesis | - |
dc.subject.keywordAuthor | epithelial-mesenchymal transition (EMT) | - |
dc.subject.keywordAuthor | gastric cancer | - |
dc.subject.keywordAuthor | ramucirumab | - |
dc.subject.keywordPlus | EPITHELIAL-MESENCHYMAL TRANSITION | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | PACLITAXEL | - |
dc.subject.keywordPlus | INITIATION | - |
dc.subject.keywordPlus | STEMNESS | - |
dc.subject.keywordPlus | LEVEL | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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