Solid Phase DNA Extraction with a Flexible Bead-Packed Microfluidic Device to Detect Methicillin-Resistant Staphylococcus aureus in Nasal Swabs
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, Kyu-Youn | - |
dc.contributor.author | Kwon, Sung Hong | - |
dc.contributor.author | Jung, Sun-Ok | - |
dc.contributor.author | Namkoong, Kak | - |
dc.contributor.author | Jung, Won-Jong | - |
dc.contributor.author | Kim, Joon-Ho | - |
dc.contributor.author | Suh, Kahp-Yang | - |
dc.contributor.author | Huh, Nam | - |
dc.date.accessioned | 2023-03-27T07:42:30Z | - |
dc.date.available | 2023-03-27T07:42:30Z | - |
dc.date.created | 2023-03-27 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/87356 | - |
dc.description.abstract | We have developed a bead-packed microfluidic device with a built-in flexible wall to automate extraction of nucleic acids from methicillin-resistant Staphylococcus aureus (MRSA) in nasal swabs. The flexible polydimethylsiloxane (PDMS) membrane was designed to manipulate the surface-to-volume ratio (SVR) of bead-packed chambers in the range of 0.05 to 0.15 (mu m(-1)) for a typical solid phase extraction protocol composed of binding, washing, and eluting. In particular, the pneumatically assisted close packing of beads led to an invariant SVR (0.15 mu m(-1)) even with different bead amounts (10-16 mg), which allowed for consistent operation of the device and improved capture efficiency for bacteria cells. Furthermore, vigorous mixing by asynchronous membrane vibration enabled ca. 90% DNA recovery with ca. mu L of liquid solution from the captured cells on the bead surfaces. The full processes to detect MRSA in nasal swabs, i.e., nasal swab collection, prefiltration, on-chip DNA extraction, and real-time polyrnerase chain reaction (PCR) amplification, were successfully constructed and carried out to validate the capability to detect MRSA in nasal swab samples. This flexible microdevice provided an excellent analytical PCR detection sensitivity of ca. 61 CFU/swab with 95% confidence interval, which turned out to be higher than or similar to that of the commercial DNA-based MRSA detection techniques. This excellent performance would be attributed to the capability of the flexible bead-packed microdevice to enrich the analyte from a large initial sample (e.g., 1 mL) into a microscale volume of eluate (e.g., 10 mu L). The proposed microdevice will find many applications as a solid phase extraction method toward various sample-to-answer systems. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.title | Solid Phase DNA Extraction with a Flexible Bead-Packed Microfluidic Device to Detect Methicillin-Resistant Staphylococcus aureus in Nasal Swabs | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000308829700048 | - |
dc.identifier.doi | 10.1021/ac3016533 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.84, no.18, pp.7912 - 7918 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84866418485 | - |
dc.citation.endPage | 7918 | - |
dc.citation.startPage | 7912 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 84 | - |
dc.citation.number | 18 | - |
dc.contributor.affiliatedAuthor | Jung, Won-Jong | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TOTAL ANALYSIS SYSTEMS | - |
dc.subject.keywordPlus | SAMPLE PREPARATION | - |
dc.subject.keywordPlus | FLOCKED SWABS | - |
dc.subject.keywordPlus | CHIPS | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | IMMUNOASSAYS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | INTEGRATION | - |
dc.subject.keywordPlus | CARRIAGE | - |
dc.subject.keywordPlus | CAPTURE | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.