The Cytoprotective Effects of Baicalein on H2O2-Induced ROS by Maintaining Mitochondrial Homeostasis and Cellular Tight Junction in HaCaT Keratinocytes
DC Field | Value | Language |
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dc.contributor.author | Kim, Gyeonghyeon | - |
dc.contributor.author | Han, Dong-Wook | - |
dc.contributor.author | Lee, Jong Hun | - |
dc.date.accessioned | 2023-06-26T15:41:03Z | - |
dc.date.available | 2023-06-26T15:41:03Z | - |
dc.date.created | 2023-06-26 | - |
dc.date.issued | 2023-04 | - |
dc.identifier.issn | 2076-3921 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/88237 | - |
dc.description.abstract | Reactive oxygen species (ROS) promote oxidative stress, which directly causes molecular damage and disrupts cellular homeostasis, leading to skin aging. Baicalein, a flavonoid compound isolated from the root of Scutellaria baicalensis Georgi has antioxidant, anticancer, anti-inflammatory, and other medicinal properties. We aimed to investigate the protective effect of baicalein on the disruption of tight junctions and mitochondrial dysfunction caused by H2O2-induced oxidative stress in HaCaT keratinocytes. The cells were pretreated with 20 and 40 mu M baicalein followed by treatment with 500 mu M H2O2. The results revealed that baicalein exerted antioxidant effects by reducing intracellular ROS production. Baicalein attenuated the degradation of the ECM (MMP-1 and Col1A1) and the disruption of tight junctions (ZO-1, occludin, and claudin-4). In addition, baicalein prevented mitochondrial dysfunction (PGC-1 alpha, PINK1, and Parkin) and restored mitochondrial respiration. Furthermore, baicalein regulated the expression of antioxidant enzymes, including NQO-1 and HO-1, via the Nrf2 signaling pathway. Our data suggest that the cytoprotective effects of baicalein against H2O2-induced oxidative stress may be mediated through the Nrf2/NQO-1/HO-1 signaling pathway. In conclusion, baicalein exerts potent antioxidant effects against H2O2-induced oxidative stress in HaCaT keratinocytes by maintaining mitochondrial homeostasis and cellular tight junctions. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | ANTIOXIDANTS | - |
dc.title | The Cytoprotective Effects of Baicalein on H2O2-Induced ROS by Maintaining Mitochondrial Homeostasis and Cellular Tight Junction in HaCaT Keratinocytes | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000980893700001 | - |
dc.identifier.doi | 10.3390/antiox12040902 | - |
dc.identifier.bibliographicCitation | ANTIOXIDANTS, v.12, no.4 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85156232804 | - |
dc.citation.title | ANTIOXIDANTS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Kim, Gyeonghyeon | - |
dc.contributor.affiliatedAuthor | Lee, Jong Hun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | baicalein | - |
dc.subject.keywordAuthor | antioxidants | - |
dc.subject.keywordAuthor | tight junction | - |
dc.subject.keywordAuthor | mitochondrial homeostasis | - |
dc.subject.keywordAuthor | Nrf2/NQO-1/HO-1 pathway | - |
dc.subject.keywordAuthor | HaCaT keratinocytes | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE | - |
dc.subject.keywordPlus | BARRIER FUNCTION | - |
dc.subject.keywordPlus | SKIN | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | DYSFUNCTION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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