Real-time monitoring of cisplatin cytotoxicity on three-dimensional spheroid tumor cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baek, N. | - |
dc.contributor.author | Seo, O.W. | - |
dc.contributor.author | Lee, J. | - |
dc.contributor.author | Hulme, J. | - |
dc.contributor.author | An, S.S.A. | - |
dc.date.available | 2020-02-28T03:44:09Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 1177-8881 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8844 | - |
dc.description.abstract | Three-dimensional (3D) cell cultivation is a powerful technique for monitoring and understanding diverse cellular mechanisms in developmental cancer and neuronal biology, tissue engineering, and drug development. 3D systems could relate better to in vivo models than two-dimensional (2D) cultures. Several factors, such as cell type, survival rate, proliferation rate, and gene and protein expression patterns, determine whether a particular cell line can be adapted to a 3D system. The 3D system may overcome some of the limitations of 2D cultures in terms of cell-cell communication and cell networks, which are essential for understanding differentiation, structural organization, shape, and extended connections with other cells or organs. Here, the effect of the anticancer drug cisplatin, also known as cis-diamminedichloroplatinum (II) or CDDP, on adenosine triphosphate (ATP) generation was investigated using 3D spheroid-forming cells and real-time monitoring for 7 days. First, 12 cell lines were screened for their ability to form 3D spheroids: prostate (DU145), testis (F9), embryonic fibroblast (NIH-3T3), muscle (C2C12), embryonic kidney (293T), neuroblastoma (SH-SY5Y), adenocarcinomic alveolar basal epithelial cell (A549), cervical cancer (HeLa), HeLa contaminant (HEp2), pituitary epithelial-like cell (GH3), embryonic cell (PA317), and osteosarcoma (U-2OS) cells. Of these, eight cell lines were selected: NIH-3T3, C2C12, 293T, SH-SY5Y, A549, HeLa, PA317, and U-2OS; and five underwent real-time monitoring of CDDP cytotoxicity: HeLa, A549, 293T, SH-SY5Y, and U-2OS. ATP generation was blocked 1 day after addition of 50 µM CDDP, but cytotoxicity in HeLa, A549, SH-SY5Y, and U-2OS cells could be visualized only 4 days after treatment. In 293T cells, CDDP failed to kill entirely the culture and ATP generation was only partially blocked after 1 day. This suggests potential CDDP resistance of 293T cells or metabolic clearance of the drug. Real-time monitoring and ATP measurements directly confirmed the cytotoxicity of CDDP, indicating that CDDP may interfere with mitochondrial activity. © 2016 Baek et al. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Dove Medical Press Ltd. | - |
dc.relation.isPartOf | Drug Design, Development and Therapy | - |
dc.title | Real-time monitoring of cisplatin cytotoxicity on three-dimensional spheroid tumor cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.doi | 10.2147/DDDT.S108004 | - |
dc.identifier.bibliographicCitation | Drug Design, Development and Therapy, v.10, pp.2155 - 2165 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85014954447 | - |
dc.citation.endPage | 2165 | - |
dc.citation.startPage | 2155 | - |
dc.citation.title | Drug Design, Development and Therapy | - |
dc.citation.volume | 10 | - |
dc.contributor.affiliatedAuthor | Hulme, J. | - |
dc.contributor.affiliatedAuthor | An, S.S.A. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | 3D spheroids | - |
dc.subject.keywordAuthor | ATP productions | - |
dc.subject.keywordAuthor | Cisplatin | - |
dc.subject.keywordAuthor | Cytotoxicity | - |
dc.subject.keywordAuthor | Real-time monitoring | - |
dc.subject.keywordPlus | adenosine triphosphate | - |
dc.subject.keywordPlus | cisplatin | - |
dc.subject.keywordPlus | adenosine triphosphate | - |
dc.subject.keywordPlus | antineoplastic agent | - |
dc.subject.keywordPlus | cisplatin | - |
dc.subject.keywordPlus | 293T cell line | - |
dc.subject.keywordPlus | 3T3 cell line | - |
dc.subject.keywordPlus | A-549 cell line | - |
dc.subject.keywordPlus | antiproliferative activity | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | C2C12 cell line | - |
dc.subject.keywordPlus | cancer resistance | - |
dc.subject.keywordPlus | cell activity | - |
dc.subject.keywordPlus | cell killing | - |
dc.subject.keywordPlus | cell structure | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | drug cytotoxicity | - |
dc.subject.keywordPlus | drug mechanism | - |
dc.subject.keywordPlus | drug monitoring | - |
dc.subject.keywordPlus | DU145 cell line | - |
dc.subject.keywordPlus | embryo | - |
dc.subject.keywordPlus | F9 cell line | - |
dc.subject.keywordPlus | female | - |
dc.subject.keywordPlus | GH3 cell line | - |
dc.subject.keywordPlus | HeLa cell line | - |
dc.subject.keywordPlus | Hep-2 cell line | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | immortalized kidney cell line | - |
dc.subject.keywordPlus | male | - |
dc.subject.keywordPlus | metabolic clearance | - |
dc.subject.keywordPlus | mitochondrion | - |
dc.subject.keywordPlus | neuroblastoma cell line | - |
dc.subject.keywordPlus | osteosarcoma cell line | - |
dc.subject.keywordPlus | PA317 cell line | - |
dc.subject.keywordPlus | prostate cancer cell line | - |
dc.subject.keywordPlus | SH SY5Y cell line | - |
dc.subject.keywordPlus | spheroid cell | - |
dc.subject.keywordPlus | teratocarcinoma cell line | - |
dc.subject.keywordPlus | tumor cell line | - |
dc.subject.keywordPlus | tumor spheroid | - |
dc.subject.keywordPlus | U 2OS cell line | - |
dc.subject.keywordPlus | cell culture technique | - |
dc.subject.keywordPlus | chemistry | - |
dc.subject.keywordPlus | time factor | - |
dc.subject.keywordPlus | Adenosine Triphosphate | - |
dc.subject.keywordPlus | Antineoplastic Agents | - |
dc.subject.keywordPlus | Cell Culture Techniques | - |
dc.subject.keywordPlus | Cisplatin | - |
dc.subject.keywordPlus | HeLa Cells | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Time Factors | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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