Chemoprevention of lotus leaf ethanolic extract through epigenetic activation of the NRF2-mediated pathway in murine skin JB6 P+ cell neoplastic transformation
DC Field | Value | Language |
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dc.contributor.author | Tung, Y.-C. | - |
dc.contributor.author | Sung, P.-H. | - |
dc.contributor.author | Chen, P.-C. | - |
dc.contributor.author | Wang, H.C. | - |
dc.contributor.author | Lee, Jong Hun | - |
dc.contributor.author | Su, Z.-Y. | - |
dc.date.accessioned | 2023-07-15T01:40:17Z | - |
dc.date.available | 2023-07-15T01:40:17Z | - |
dc.date.created | 2023-06-16 | - |
dc.date.issued | 2023-07 | - |
dc.identifier.issn | 2225-4110 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/88501 | - |
dc.description.abstract | Background and aim: Skin is one barrier protecting from environmental risk factors that can make skin cells cancerous through DNA damage and oxidative stress. The nuclear factor erythroid 2-related factor 2 (NRF2) pathway is an anti-stress defense system that can be regulated by DNA methylation and histone modification. Dietary phytochemicals have chemopreventive properties that can inhibit or delay carcinogenesis. The lotus leaf is a traditional medicinal plant containing many polyphenols whose extracts show many biological activities, including antioxidant, anti-obesity, and anti-cancer. This study aim to investigate the effect of lotus leaves on neoplastic transformation in murine skin JB6 P+ cells. Experimental procedure: Lotus leaves were extracted with water (LL-WE) and ethanol (LL-EE), and the LL-WE residues were further extracted with ethanol (LL-WREE). JB6 P+ cells were treated with different extracts. The chemoprotective effect would be evaluated by heme oxygenase 1 (HO-1), NAD(P)H quinone oxidoreductase (NQO1), and UDP glucuronosyltransferase family 1 member A1 (UGT1A1) expression. Results and conclusion: LL-EE contained higher total phenolics and quercetin among extracts. In mouse skin JB6 P+ cells with 12-O-tetradecanoylphorbol-13-acetate treatment, LL-EE showed the greatest potential to suppress skin carcinogenesis. LL-EE activated the NRF2 pathway by upregulating antioxidant and detoxification enzymes upregulates antioxidant and detoxification enzymes, including HO-1, NQO1, and UGT1A1, and downregulates DNA methylation, which might be caused by lower DNA methyltransferase and histone deacetylase levels. Therefore, our results show that LL-EE reduces the neoplastic transformation of skin JB6 P+ cells, potentially by activating the NRF2 pathway and regulating epigenetic DNA methylation and histone acetylation. © 2023 Center for Food and Biomolecules, National Taiwan University | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | National Taiwan University | - |
dc.relation.isPartOf | Journal of Traditional and Complementary Medicine | - |
dc.title | Chemoprevention of lotus leaf ethanolic extract through epigenetic activation of the NRF2-mediated pathway in murine skin JB6 P+ cell neoplastic transformation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 001021595800001 | - |
dc.identifier.doi | 10.1016/j.jtcme.2023.02.002 | - |
dc.identifier.bibliographicCitation | Journal of Traditional and Complementary Medicine, v.13, no.4, pp.337 - 344 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85150343513 | - |
dc.citation.endPage | 344 | - |
dc.citation.startPage | 337 | - |
dc.citation.title | Journal of Traditional and Complementary Medicine | - |
dc.citation.volume | 13 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Lee, Jong Hun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Antioxidant | - |
dc.subject.keywordAuthor | Cancer transformation | - |
dc.subject.keywordAuthor | DNA methylation | - |
dc.subject.keywordAuthor | Herbal medicine | - |
dc.subject.keywordAuthor | Skin cell | - |
dc.subject.keywordPlus | NRF2 | - |
dc.subject.keywordPlus | PHOTOPROTECTION | - |
dc.subject.keywordPlus | POLYPHENOLS | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | TISSUES | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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