Exogenous 8-Hydroxydeoxyguanosine Attenuates PM2.5-Induced Inflammation in Human Bronchial Epithelial Cells by Decreasing NLRP3 Inflammasome Activation
DC Field | Value | Language |
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dc.contributor.author | Bang, Jihye | - |
dc.contributor.author | Son, Kuk Hui | - |
dc.contributor.author | Heo, Hye-Ryeon | - |
dc.contributor.author | Park, Eunsook | - |
dc.contributor.author | Kwak, Hyun-Jeong | - |
dc.contributor.author | Uhm, Kyung-Ok | - |
dc.contributor.author | Chung, Myung-Hee | - |
dc.contributor.author | Kim, Young-Youl | - |
dc.contributor.author | Lim, Hyun Joung | - |
dc.date.accessioned | 2023-07-21T04:40:49Z | - |
dc.date.available | 2023-07-21T04:40:49Z | - |
dc.date.created | 2023-07-21 | - |
dc.date.issued | 2023-06 | - |
dc.identifier.issn | 2076-3921 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/88546 | - |
dc.description.abstract | Particulate matter 2.5 (PM2.5) induces lung injury by increasing the generation of reactive oxygen species (ROS) and inflammation. ROS aggravates NLRP3 inflammasome activation, which activates caspase-1, IL-1 & beta;, and IL-18 and induces pyroptosis; these factors propagate inflammation. In contrast, treatment with exogenous 8-hydroxydeoxyguanosine (8-OHdG) decreases RAC1 activity and eventually decreases dinucleotide phosphate oxidase (NOX) and ROS generation. To establish modalities that would mitigate PM2.5-induced lung injury, we evaluated whether 8-OHdG decreased PM2.5-induced ROS generation and NLRP3 inflammasome activation in BEAS-2B cells. CCK-8 and lactate dehydrogenase assays were used to determine the treatment concentration. Fluorescence intensity, Western blotting, enzyme-linked immunosorbent assay, and immunoblotting assays were also performed. Treatment with 80 & mu;g/mL PM2.5 increased ROS generation, RAC1 activity, NOX1 expression, NLRP3 inflammasome (NLRP3, ASC, and caspase-1) activity, and IL-1 & beta; and IL-18 levels in cells; treatment with 10 & mu;g/mL 8-OHdG significantly attenuated these effects. Furthermore, similar results, such as reduced expression of NOX1, NLRP3, ASC, and caspase-1, were observed in PM2.5-treated BEAS-2B cells when treated with an RAC1 inhibitor. These results show that 8-OHdG mitigates ROS generation and NLRP3 inflammation by inhibiting RAC1 activity and NOX1 expression in respiratory cells exposed to PM2.5. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | ANTIOXIDANTS | - |
dc.title | Exogenous 8-Hydroxydeoxyguanosine Attenuates PM2.5-Induced Inflammation in Human Bronchial Epithelial Cells by Decreasing NLRP3 Inflammasome Activation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 001016860400001 | - |
dc.identifier.doi | 10.3390/antiox12061189 | - |
dc.identifier.bibliographicCitation | ANTIOXIDANTS, v.12, no.6 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85163820455 | - |
dc.citation.title | ANTIOXIDANTS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Son, Kuk Hui | - |
dc.contributor.affiliatedAuthor | Chung, Myung-Hee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | particulate matter 2 | - |
dc.subject.keywordAuthor | 5 | - |
dc.subject.keywordAuthor | lung injury | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | 8-hydroxydeoxyguanosine | - |
dc.subject.keywordAuthor | NLRP3 inflammasome | - |
dc.subject.keywordPlus | AIR-POLLUTION | - |
dc.subject.keywordPlus | RHO-GTPASES | - |
dc.subject.keywordPlus | GASDERMIN-D | - |
dc.subject.keywordPlus | RAC1 | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordPlus | PYROPTOSIS | - |
dc.subject.keywordPlus | PM2.5 | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | CASPASES | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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