Phosphorylation of tyrosine-14 on Caveolin-1 enhances lipopolysaccharide-induced inflammation in human intestinal Caco-2 cells
DC Field | Value | Language |
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dc.contributor.author | Bae, Gong Deuk | - |
dc.contributor.author | Kim, Kyong | - |
dc.contributor.author | Jang, Se-Eun | - |
dc.contributor.author | Baek, Dong-Jae | - |
dc.contributor.author | Park, Eun-Young | - |
dc.contributor.author | Oh, Yoon Sin | - |
dc.date.accessioned | 2023-08-08T08:40:23Z | - |
dc.date.available | 2023-08-08T08:40:23Z | - |
dc.date.created | 2023-08-02 | - |
dc.date.issued | 2023-12 | - |
dc.identifier.issn | 1976-0442 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/88705 | - |
dc.description.abstract | Caveolin-1 (Cav-1) is the main structural component of the caveolae on the plasma membrane, which regulates various cellular processes, including cell growth, differentiation, and endocytosis. Although a recent study demonstrated that Cav-1 might be involved in diabetes-associated inflammation, its exact role in the intestine was unclear. In this study, we examined the intestinal expression of Cav-1 in diabetic conditions. We also investigated its effect on lipopolysaccharide (LPS)-induced inflammation by expressing this protein in human intestinal Caco- 2 cells lacking Cav-1. We observed that increased Cav-1 levels and decreased expression of tight junction proteins affected intestinal permeability in high-fat diet-induced diabetic mice. When Caco-2 cells were treated with LPS, Cav-1 enhanced the NF-κB signaling. Moreover, LPS reduced the expression of tight junction proteins while it increased cell-cell permeability and reactive oxygen species generation in Caco-2 cells and this effect was amplified by cav-1 overexpression. LPS treatment promoted phosphorylation of tyrosine-14 (Y14) on Cav-1, and the LPSinduced NF-κB signaling was suppressed in cells expressing nonphosphorylatable Cav-1 (tyrosine-14 to phenylalanine mutant), which reduced intestinal barrier permeability. These results suggest that Cav-1 expression promotes LPS-induced inflammation in Caco-2 cells, and phosphorylation of Y14 on Cav-1 might contribute to the anti-inflammatory response in LPS-induced NF- κB signaling and cell permeability. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 한국응용생명화학회 | - |
dc.relation.isPartOf | Journal of Applied Biological Chemistry | - |
dc.title | Phosphorylation of tyrosine-14 on Caveolin-1 enhances lipopolysaccharide-induced inflammation in human intestinal Caco-2 cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.doi | 10.3839/jabc.2023.043 | - |
dc.identifier.bibliographicCitation | Journal of Applied Biological Chemistry, v.66, pp.311 - 319 | - |
dc.identifier.kciid | ART002981568 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85166072293 | - |
dc.citation.endPage | 319 | - |
dc.citation.startPage | 311 | - |
dc.citation.title | Journal of Applied Biological Chemistry | - |
dc.citation.volume | 66 | - |
dc.contributor.affiliatedAuthor | Bae, Gong Deuk | - |
dc.subject.keywordAuthor | Caveolin-1 · High fat diet · Intestine · Permeability · Tight junction protein | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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