Method to Determine the Optimal Aptamer-to-Bead Ratio by Using Flow Cytometry
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Sun Young | - |
dc.contributor.author | Kim, Eun-Ok | - |
dc.contributor.author | Jang, Daehyuk | - |
dc.contributor.author | Hwang, Soonjae | - |
dc.contributor.author | Rhee, Ki-Jong | - |
dc.contributor.author | Yun, Miyong | - |
dc.date.accessioned | 2023-08-17T06:40:40Z | - |
dc.date.available | 2023-08-17T06:40:40Z | - |
dc.date.created | 2023-08-17 | - |
dc.date.issued | 2023-07 | - |
dc.identifier.issn | 2090-908X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/88770 | - |
dc.description.abstract | Research on the effective attachment of aptamers to beads, which is essential for using aptamers, has made relatively little progress. Here, we demonstrate a new method based on flow cytometry to determine the optimal aptamer-to-bead ratio for aptamer immobilization. The fluorescence intensity increased with a gradual two-fold increase in the aptamer fluorescence concentration, peaked at an aptamer-to-bead ratio of 2.56 x 10(5), and tended to decrease at higher ratios. A similar pattern was observed in an additional analysis using fluorescence microscopy. However, measurement of the free aptamer concentration after the aptamer-bead conjugation reaction revealed a large aptamer loss compared to the 1.28 x 10(5) aptamer-bead ratio. In addition, the binding efficiency of the aptamer/bead to the target was highest at the aptamer-to-bead ratio of 1.28 x 10(5). Taken together, our data suggest that the proposed method is the best and easiest for determining the optimal aptamer-to-bead ratio. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | HINDAWI LTD | - |
dc.relation.isPartOf | SCIENTIFICA | - |
dc.title | Method to Determine the Optimal Aptamer-to-Bead Ratio by Using Flow Cytometry | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 001031408200001 | - |
dc.identifier.doi | 10.1155/2023/5842652 | - |
dc.identifier.bibliographicCitation | SCIENTIFICA, v.2023 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85165929758 | - |
dc.citation.title | SCIENTIFICA | - |
dc.citation.volume | 2023 | - |
dc.contributor.affiliatedAuthor | Hwang, Soonjae | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | IN-VITRO SELECTION | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.