바이칼레인(baicalein)이 poly-IC와 lipoteichoic acid로 자극된 마우스 대식세포 RAW 264.7의 hydrogen peroxide 생성에 미치는 영향
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박완수 | - |
dc.date.accessioned | 2023-08-29T00:41:04Z | - |
dc.date.available | 2023-08-29T00:41:04Z | - |
dc.date.created | 2023-08-28 | - |
dc.date.issued | 2023-07 | - |
dc.identifier.issn | 1229-1765 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/88942 | - |
dc.description.abstract | Objectives : The aim of this study was to investigate the effect of baicalein (BA) on the production of hydrogen peroxide and nitric oxide (NO) in RAW 264.7 mouse macrophages stimulated with polyinosinic-polycytidylic acid (poly-IC) and lipoteichoic acid. Methods : RAW 264.7 co-stimulated with poly-IC and lipoteichoic acid were incubated with baicalein at concentrations of 25 and 50 μM. Incubation time is 16 h, 18 h, 20 h, 22 h, and 24 h. After incubation, The production of hydrogen peroxide in RAW 264.7 was measured with dihydrorhodamine 123 assay. Chrysin was used as a comparative material. NO production was evaluated by griess assay. Results : For 16 h, 18 h, 20 h, 22 h, and 24 h incubation, BA at the concentration of 25 and 50 μM significantly inhibited the production of hydrogen peroxide in RAW 264.7 stimulated by poly-IC and lipoteichoic acid (p <0.001). In details, production of hydrogen peroxide in ‘poly-IC and lipoteichoic acid’-stimulated RAW 264.7 treated for 16 h with BA at concentrations of 25 and 50 μM was 82.36% and 77.24% of the control group treated with poly-IC and lipoteichoic acid only, respectively; the production of hydrogen peroxide for 18 h was 83.15% and 77.91%, respectively; production of hydrogen peroxide for 20 h was 82.88% and 77.82%, respectively; production of hydrogen peroxide for 22 h was 83.27% and 78.17%, respectively; production of hydrogen peroxide for 24 h was 83.54% and 78.35%, respectively. Additionally, BA at the concentration of 50 and 100 μM significantly inhibited NO production in lipoteichoic acid-induced RAW 264.7 (p <0.001). Conclusions : BA might have anti-oxidative activity related to its inhibition of hydrogen peroxide production in ‘poly- IC and lipoteichoic acid’-stimulated RAW 264.7 macrophages. | - |
dc.language | 한국어 | - |
dc.language.iso | ko | - |
dc.publisher | 대한본초학회 | - |
dc.relation.isPartOf | 대한본초학회지 | - |
dc.title | 바이칼레인(baicalein)이 poly-IC와 lipoteichoic acid로 자극된 마우스 대식세포 RAW 264.7의 hydrogen peroxide 생성에 미치는 영향 | - |
dc.title.alternative | Effects of baicalein on hydrogen peroxide productions in RAW 264.7 mouse macrophages stimulated by poly-IC and lipoteichoic acid | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | 대한본초학회지, v.38, no.4, pp.11 - 19 | - |
dc.identifier.kciid | ART002982151 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 19 | - |
dc.citation.startPage | 11 | - |
dc.citation.title | 대한본초학회지 | - |
dc.citation.volume | 38 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | 박완수 | - |
dc.subject.keywordAuthor | RAW 264.7 | - |
dc.subject.keywordAuthor | Baicalein | - |
dc.subject.keywordAuthor | Macrophage | - |
dc.subject.keywordAuthor | poly-IC | - |
dc.subject.keywordAuthor | Lipoteichoic acid | - |
dc.subject.keywordAuthor | Hydrogen peroxide | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.