A CRISPR-Cas12a-Based Diagnostic Method for Japanese Encephalitis Virus Genotypes I, III, and V
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwak, Namki | - |
dc.contributor.author | Park, Bum Ju | - |
dc.contributor.author | Song, Yoon-Jae | - |
dc.date.accessioned | 2023-09-15T14:40:23Z | - |
dc.date.available | 2023-09-15T14:40:23Z | - |
dc.date.created | 2023-09-15 | - |
dc.date.issued | 2023-08 | - |
dc.identifier.issn | 2079-6374 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/89091 | - |
dc.description.abstract | The Japanese encephalitis virus (JEV) is prevalent in Asian countries, including Korea, Japan, China, Vietnam, and India. JEV is transmitted to humans by Culex mosquitoes. Despite extensive research efforts, no approved antiviral agents are currently available, although JE can be prevented by vaccination. DNA endonuclease-targeted CRISPR trans reporter (DETECTR) is a newly emerging CRISPR-Cas12a-based molecular diagnostic method combined with isothermal nucleic acid amplification. In this study, DETECTR with reverse transcription-recombinase polymerase amplification (RT-RPA) was effectively utilized for JEV diagnosis and detected down to 10 RNA copies for JEV genotype I (GI) and 1 x 10(2) copies for both GIII and GV, achieving similar sensitivity to RT-PCR while displaying no cross-reaction with other viruses. A one-tube, one-temperature format of DETECTR was further developed, and its efficiency compared with that of conventional DETECTR. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | BIOSENSORS-BASEL | - |
dc.title | A CRISPR-Cas12a-Based Diagnostic Method for Japanese Encephalitis Virus Genotypes I, III, and V | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 001055794000001 | - |
dc.identifier.doi | 10.3390/bios13080769 | - |
dc.identifier.bibliographicCitation | BIOSENSORS-BASEL, v.13, no.8 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85168738083 | - |
dc.citation.title | BIOSENSORS-BASEL | - |
dc.citation.volume | 13 | - |
dc.citation.number | 8 | - |
dc.contributor.affiliatedAuthor | Kwak, Namki | - |
dc.contributor.affiliatedAuthor | Park, Bum Ju | - |
dc.contributor.affiliatedAuthor | Song, Yoon-Jae | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Japanese encephalitis virus | - |
dc.subject.keywordAuthor | diagnosis | - |
dc.subject.keywordAuthor | CRISPR-Cas12a | - |
dc.subject.keywordAuthor | DETECTR | - |
dc.subject.keywordPlus | RT-PCR ASSAY | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.