N-retinylidene-N-retinylethanolamine degradation in human retinal pigment epithelial cells via memantine- and ifenprodil-mediated autophagy
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jae Rim | - |
dc.contributor.author | Jeong, Kwang Won | - |
dc.date.accessioned | 2023-10-09T01:40:41Z | - |
dc.date.available | 2023-10-09T01:40:41Z | - |
dc.date.created | 2023-09-24 | - |
dc.date.issued | 2023-09 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/89219 | - |
dc.description.abstract | N-methyl-D-aspartate (NMDA) receptors are ionic glutamine receptors involved in brain development and functions such as learning and memory forma-tion. NMDA receptor inhibition is associated with autophagy activation. In this study, we investigated whether the NMDA receptor antagonists, memantine and ifenprodil, induce autophagy in human retinal pigment epithelial cells (ARPE-19) to remove N-retinylidene-N-retinylethanolamine (A2E), an intracellular lipofuscin component. Flu-orometric analysis using labeled A2E (A2E-BDP) and confocal microscopic examina-tion revealed that low concentrations of NMDA receptor antagonists, which did not induce cytotoxicity, significantly reduced A2E accumulation in ARPE-19 cells. In addi-tion, memantine and ifenprodil activated autophagy in ARPE-19 cells as measured by microtubule-associated protein 1A/1B-light chain3-II formation and phosphorylated p62 protein levels. Further, to understand the correlation between memantine-and ifenprodil-mediated A2E degradation and autophagy, autophagy-related 5 (ATG5) was depleted using RNA interference. Memantine and ifenprodil failed to degrade A2E in ARPE-19 cells lacking ATG5. Taken together, our study indicates that the NMDA receptor antagonists, memantine and ifenprodil, can remove A2E accumulated in cells via autophagy activation in ARPE-19 cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | The Korean Physiological Society and The Korean Society of Pharmacology | - |
dc.relation.isPartOf | The Korean Journal of Physiology & Pharmacology | - |
dc.title | N-retinylidene-N-retinylethanolamine degradation in human retinal pigment epithelial cells via memantine- and ifenprodil-mediated autophagy | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 001062637500003 | - |
dc.identifier.doi | 10.4196/kjpp.2023.27.5.449 | - |
dc.identifier.bibliographicCitation | The Korean Journal of Physiology & Pharmacology, v.27, no.5, pp.449 - 456 | - |
dc.identifier.kciid | ART002990801 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85173501818 | - |
dc.citation.endPage | 456 | - |
dc.citation.startPage | 449 | - |
dc.citation.title | The Korean Journal of Physiology & Pharmacology | - |
dc.citation.volume | 27 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | Lee, Jae Rim | - |
dc.contributor.affiliatedAuthor | Jeong, Kwang Won | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | A2E | - |
dc.subject.keywordAuthor | ARPE-19 | - |
dc.subject.keywordAuthor | Autophagy | - |
dc.subject.keywordAuthor | Ifenprodil | - |
dc.subject.keywordAuthor | Memantine | - |
dc.subject.keywordPlus | RPE LIPOFUSCIN | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | ATG16L | - |
dc.subject.keywordPlus | SITE | - |
dc.subject.keywordPlus | A2E | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.