Complete genome sequence of an Acute Hepatopancreatic Necrosis Disease (AHPND)-causing Vibrio parahaemolyticus 19-021-D1 isolated from cultured pacific white shrimp (Penaeus vannamei) at 2019 in Korea

Abstract

Vibrio parahaemolyticus is one of the most important seafoodborne pathogens that cause several life-threatening infections or large food poisoning outbreaks. Some strains of species harboring the pVA1-type plasmid containing the two toxin subunit genes (pirA and pirB) are the major etiological agents of acute hepatopancreatic necrosis disease (AHPND), causing significant economic losses in the global shrimp aquaculture industry. Previously, we reported the first complete genome of the pVA1-type plasmid (pVp_Kor-D1-2) of V. parahaemolyticus 19-021-D1, which caused a severe AHPND outbreak in cultured pacific white shrimp (Penaeus vannamei) at 2019 in Korea. Here, we present the whole genomic characteristics of the bacterial chromosomes and plasmids of strain 19-021-D1. The genome consisted of two circular chromosomes and two plasmids (including pVp_Kor-D1-1 and pVp_Kor-D1-2). Several genes encoding virulence, antimicrobial resistance, and prophage regions were detected in the 19-021-D1 genome. Multilocus sequence typing analyses identified the sequence type of strain 19-021-D1 as 413 and revealed that the Korean isolate may be associated with those collected from cultured shrimp from different geographical regions. This genomic information provides important insights into control strategies against the prevalent AHPND-causing V. parahaemolyticus in Korean aquaculture and seafood industries. © 2023, The Microbiological Society of Korea