Systemic multiomics evaluation of the therapeutic effect of Bacteroides species on liver cirrhosis in male mice
DC Field | Value | Language |
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dc.contributor.author | Park, Ye Rin | - |
dc.contributor.author | Lee, Hae Lee | - |
dc.contributor.author | Hyun, Ji Ye | - |
dc.contributor.author | Choi, Jieun | - |
dc.contributor.author | Moon, Ji Hyun | - |
dc.contributor.author | Kim, Byung Yong | - |
dc.contributor.author | Yang, Seung-Jo | - |
dc.contributor.author | Lee, Je Hee | - |
dc.contributor.author | Kim, Byoung Kook | - |
dc.contributor.author | Park, Tae-Sik | - |
dc.contributor.author | Suk, Ki Tae | - |
dc.contributor.author | Lee, Do Yup | - |
dc.date.accessioned | 2023-12-15T15:10:32Z | - |
dc.date.available | 2023-12-15T15:10:32Z | - |
dc.date.issued | 2023-10 | - |
dc.identifier.issn | 2165-0497 | - |
dc.identifier.issn | 2165-0497 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/89610 | - |
dc.description.abstract | The human gut microbiome is engaged in biological homeostasis in the gut-liver axis and across multi-organs. The aim of this study is to investigate the therapeutic effects of human gut-derived microbes, Bacteroides species on liver cirrhosis in a mouse model. The experiment was performed on male mice, which were divided into five groups: normal control (NC), disease control, Bacteroides dorei-, Bacteroides cellulosilyticus-, and ursodeoxycholic acid-supplemented groups after 3,5-diethoxycarbonyl-1,4-dihydrocollidine treatment. The therapeutic effect was evaluated based on liver physiology and the expression level of hepatic fibrosis. Untargeted and targeted metabolic profiling was conducted on cecal, fecal, liver, and serum samples using ultra-performance liquid-chromatography coupled with high-resolution mass-spectrometry. The gut microbial taxonomic composition was analyzed by 16S rRNA gene amplicon sequencing from the stool of each mice group. The Bacteroides treatment improved the liver/body weight ratio and normalized hepatic fibrosis biomarkers, including COL1A1. The fecal metabolome showed the most distinctive and characteristic profiles according to different treatments, compared to other sample matrices (cecum, liver, and blood). Key metabolites were identified, which indicated the potential therapeutic effect of the B. dorei treatment. Among them, a short-chain fatty acid, propionic acid, showed consistent upregulation in the cecum and liver after the B. dorei treatment. Microbiome analysis showed that Akkermansia muciniphila was retained in the group treated with B. dorei at a similar level as in the NC group. Our current multiomics study of systemic dynamics demonstrated that Bacteroides species, particularly B. dorei, ameliorated liver cirrhosis by modulating the metabolic and microbial environment to the normal state within the gut-liver axis. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Systemic multiomics evaluation of the therapeutic effect of Bacteroides species on liver cirrhosis in male mice | - |
dc.type | Article | - |
dc.identifier.wosid | 001082279200001 | - |
dc.identifier.doi | 10.1128/spectrum.05349-22 | - |
dc.identifier.bibliographicCitation | MICROBIOLOGY SPECTRUM, v.11, no.6, pp e0534922 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85180013512 | - |
dc.citation.startPage | e0534922 | - |
dc.citation.title | MICROBIOLOGY SPECTRUM | - |
dc.citation.volume | 11 | - |
dc.citation.number | 6 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | liver cirrhosis | - |
dc.subject.keywordAuthor | Bacteroides | - |
dc.subject.keywordAuthor | multiomics | - |
dc.subject.keywordAuthor | gut-liver axis | - |
dc.subject.keywordAuthor | metabolomics | - |
dc.subject.keywordAuthor | microbiome | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PROBIOTICS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | ACID | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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