A liquid crystal-based biosensor for sensitive detection of tumor necrosis factor-alpha
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryu, Je-Jin | - |
dc.contributor.author | Jang, Chang-Hyun | - |
dc.date.accessioned | 2024-01-24T12:00:17Z | - |
dc.date.available | 2024-01-24T12:00:17Z | - |
dc.date.issued | 2024-01 | - |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.issn | 1436-5073 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/90215 | - |
dc.description.abstract | Tumor necrosis factor-alpha (TNF-alpha) is a cytokine secreted by the macrophages and Th1 cells of the immune system in response to inflammation. Given its significance as a biomarker with elevated levels in physiological fluids in various conditions, there is an increasing demand for a simple and accurate TNF-alpha detection strategy. In this article, we present a liquid crystal (LC)-based biosensor developed for sensitive TNF-alpha detection. The biosensor operates as follows: TNF-alpha and detection antibodies (DAbs) form complexes during preincubation. These complexes then bind with the surface-immobilized capture antibodies (CAbs), facilitating the antigen-antibody reaction between the CAbs and the TNF-alpha/DAb complexes. This target recognition interaction alters the surface topography, disrupting the vertical orientation of LCs produced by dimethyloctadecyl[3-(trimethoxysilyl)-propyl]ammonium chloride. The orientational change in the LCs can be easily visualized with a polarized optical microscope, resulting in brighter images as TNF-alpha levels rise. Our results demonstrated a linear range of 5.00-500 pg/mL, with a limit of detection and limit of quantification being 1.08 and 3.56 pg/mL, respectively. Recovery experiments on diluted saliva samples produced reasonable results, with TNF-alpha recoveries ranging from 97.1% +/- 2.58% to 107% +/- 5.95%. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER WIEN | - |
dc.title | A liquid crystal-based biosensor for sensitive detection of tumor necrosis factor-alpha | - |
dc.type | Article | - |
dc.identifier.wosid | 001131879600002 | - |
dc.identifier.doi | 10.1007/s00604-023-06125-y | - |
dc.identifier.bibliographicCitation | MICROCHIMICA ACTA, v.191, no.1 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85180657512 | - |
dc.citation.title | MICROCHIMICA ACTA | - |
dc.citation.volume | 191 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.publisher.location | 오스트리아 | - |
dc.subject.keywordAuthor | Liquid crystal | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | Tumor necrosis factor-alpha | - |
dc.subject.keywordAuthor | Antibody | - |
dc.subject.keywordAuthor | Polarized optical microscope | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | ELECTROCHEMICAL BIOSENSOR | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | IMMUNOSENSOR | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | SALIVA | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordPlus | IL-6 | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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