Alleviative Effect of Geniposide on Lipopolysaccharide-Stimulated Macrophages via Calcium Pathway
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyun-Ju | - |
dc.contributor.author | Park, Wansu | - |
dc.date.accessioned | 2024-03-11T12:00:29Z | - |
dc.date.available | 2024-03-11T12:00:29Z | - |
dc.date.issued | 2024-02 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/90636 | - |
dc.description.abstract | In this study, we investigated how geniposide (a bioactive ingredient of gardenia fruit) acts on lipopolysaccharide (LPS)-stimulated macrophages. Griess reagent assay, Fluo-4 calcium assay, dihydrorhodamine 123 assay, multiplex cytokine assay, quantitative RT-PCR, and flow cytometry assay were used for this study. Data showed that geniposide at concentrations of 10, 25, and 50 mu M reduced significantly the levels of nitric oxide, intracellular Ca2+, and hydrogen peroxide in LPS-activated RAW 264.7. Multiplex cytokine assay showed that geniposide at concentrations of 10, 25, and 50 mu M meaningfully suppressed levels of IL-6, G-CSF, MCP-1, and MIP-1 alpha in RAW 264.7 provoked by LPS; additionally, geniposide at concentrations of 25 and 50 mu M meaningfully suppressed the levels of TNF-alpha, IP-10, GM-CSF, and MIP-1 beta. Flow cytometry assay showed that geniposide reduces significantly the level of activated P38 MAPK in RAW 264.7 provoked by LPS. Geniposide meaningfully suppressed LPS-induced transcription of inflammatory target genes, such as Chop, Jak2, Fas, c-Jun, c-Fos, Stat3, Nos2, Ptgs2, Gadd34, Asc, Xbp1, Nlrp3, and Par-2. Taken together, geniposide exerts alleviative effects in LPS-stimulated macrophages via the calcium pathway. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | Alleviative Effect of Geniposide on Lipopolysaccharide-Stimulated Macrophages via Calcium Pathway | - |
dc.type | Article | - |
dc.identifier.wosid | 001160355500001 | - |
dc.identifier.doi | 10.3390/ijms25031728 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.25, no.3 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85184703486 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 25 | - |
dc.citation.number | 3 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | geniposide | - |
dc.subject.keywordAuthor | lipopolysaccharide | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | macrophages | - |
dc.subject.keywordAuthor | cytokine | - |
dc.subject.keywordAuthor | calcium | - |
dc.subject.keywordAuthor | P38 MAPK | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | GARDENIA-JASMINOIDES | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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