Proanthocyanidins and Phenolic Compounds from the Twigs of Salix chaenomeloides and Their Anti-Lipogenic Effects on 3T3-L1 Preadipocytes
DC Field | Value | Language |
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dc.contributor.author | Kim, Kyung Ah | - |
dc.contributor.author | Tran, Nguyen Khoi Song | - |
dc.contributor.author | Baek, Jiwon | - |
dc.contributor.author | Lee, Soah | - |
dc.contributor.author | Kang, Ki Sung | - |
dc.contributor.author | Kim, Ki Hyun | - |
dc.date.accessioned | 2024-06-18T14:00:24Z | - |
dc.date.available | 2024-06-18T14:00:24Z | - |
dc.date.issued | 2024-04 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/91558 | - |
dc.description.abstract | The present study investigated potential bioactive natural products from the EtOH extract of Salix chaenomeloides twigs using column chromatography, leading to the isolation of six compounds (1-6), which were characterized as two proanthocyanidins, procyanidin B-2 (1) and procyanidin B-1 (2), and four phenolic compounds, 4-hydroxybenzoic acid beta-D-glucosyl ester (3), di-O-methylcrenatin (4), p-coumaric acid glucoside (5), and syringin (6) by the comparison of their NMR spectra with the reported data and high-resolution (HR)-electrospray ionization mass spectroscopy (ESI-MS) analysis. We investigated the potential of six compounds (1-6) to inhibit adipogenesis in 3T3-L1 preadipocytes, which showed that the compounds (1-6) significantly reduced lipid accumulation in 3T3-L1 adipocytes without affecting cell proliferation. Notably, compound 1 demonstrated a remarkable 60% and 90% reduction in lipid levels with 50 and 100 mu M treatments, respectively. Oil Red O staining results indicated that compound 1 significantly inhibits the formation of lipid droplets, comparable to the effect of T863, an inhibitor of triglyceride used as a positive control, in adipocytes. Compound 1 had no effect on the regulators PPAR gamma, C/EBP alpha, and SREBF1 of adipocyte differentiation in 3T3-L1 preadipocytes, but compound 1 activated the fatty acid oxidation regulator, PPAR alpha, compared to the lipogenic-induced control. It also suppressed fatty acid synthesis by downregulating the expression of fatty acid synthase (FAS). Finally, compound 1 induced the mRNA and protein levels of CPT1A, an initial marker of mitochondrial fatty acid oxidation in 3T3-L1. This finding substantiates the anti-lipogenic and lipolytic effects of procyanidin B-2 (1) in 3T3-L1 preadipocytes, emphasizing its pivotal role in modulating obesity-related markers. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | Proanthocyanidins and Phenolic Compounds from the Twigs of Salix chaenomeloides and Their Anti-Lipogenic Effects on 3T3-L1 Preadipocytes | - |
dc.type | Article | - |
dc.identifier.wosid | 001201648100001 | - |
dc.identifier.doi | 10.3390/nu16071036 | - |
dc.identifier.bibliographicCitation | NUTRIENTS, v.16, no.7 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-85190492944 | - |
dc.citation.title | NUTRIENTS | - |
dc.citation.volume | 16 | - |
dc.citation.number | 7 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | Salix chaenomeloides | - |
dc.subject.keywordAuthor | HPLC | - |
dc.subject.keywordAuthor | NMR | - |
dc.subject.keywordAuthor | procyanidin B-2 | - |
dc.subject.keywordAuthor | fatty acid oxidation | - |
dc.subject.keywordAuthor | anti-lipogenesis | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | BARK | - |
dc.subject.keywordPlus | FAT | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | DERIVATIVES | - |
dc.subject.keywordPlus | GLUCOSIDES | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.subject.keywordPlus | LEAVES | - |
dc.subject.keywordPlus | CELLS | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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