Synergistic Effect of Natural Killer Cells and Bee Venom on Inhibition of NCI-H157 Cell Growth
- Authors
- 성희진; 송호섭
- Issue Date
- 2016
- Publisher
- 대한침구의학회
- Keywords
- Bee Venom; Lung Cancer; Natural Killer cell; Death Receptor; Nuclear Factor-κB; Apoptosis
- Citation
- Journal of Acupuncture Research, v.33, no.1, pp.47 - 56
- Journal Title
- Journal of Acupuncture Research
- Volume
- 33
- Number
- 1
- Start Page
- 47
- End Page
- 56
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9546
- ISSN
- 2586-288X
- Abstract
- Objectives : This study examined the effects of Bee venom on apoptosis in NCI-H157 human lung cancer cells and for promoting the apoptosis effects of Natural killer cell.
Methods: Bee venom and Natural killer-92 cells were cultured either separately from or together with NCI-H157 cells for 24 hours. To figure out whether Bee venom enhances the cytotoxic ef- fect of Natural Killer-92 cells, a cell viability assay was conducted. To observe the changes in Death receptors, apoptotic regulatory proteins and Nuclear factor-κB, western blot analysis was conducted. To observe the effect of Bee venom through an extrinsic mechanism, a trans- fection assay was conducted.
Results: 1. Natural killer-92 cells and Bee venom significantly inhibited the growth of NCI-H157 cells and co-culture had more inhibitory effect than the separate culture.
2. Expressions of Fas, DR3, DR6, Bax, caspase-3, caspase-8, cleaved caspase-3, cleaved caspase-8 were increased, and expressions of Bcl-2 and cIAP were decreased. More ef- ficacy was observed in co-culture than in separate culture.
3. Nuclear Factor-κB activation was clearly decreased. And co-culture showed much less activation than separate culture.
4. As a result of treatment for DR-siRNA, the reduced cell viability of NCI-H157 cells and the activity of Nuclear factor-κB were increased. With this, it can be seen that Bee venom and Natural killer-92 cells have an effect on the cancer cells through the extrinsic mechanism. Conclusion : Bee venom is effective in inhibiting the growth of human lung cancer cells. Fur- thermore Bee venom effectively enhances the functions of Natural killer cells.
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