Label-free simple fluorescence assay of DNA polymerase using the G-quadruplex structure
DC Field | Value | Language |
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dc.contributor.author | Kim, Seyeon | - |
dc.contributor.author | Gang, Jongback | - |
dc.date.available | 2020-02-28T06:43:15Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2016-01 | - |
dc.identifier.issn | 1759-9660 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9687 | - |
dc.description.abstract | In this study, the G-quadruplex structure was used to detect the enzymatic activity and inhibition of the Klenow fragment exo(-) (KF-) in the presence of N-methyl mesoporphyrin IX (NMM) which binds specifically to the G-quadruplex. We found that KF- synthesized complementary strands of the template DNA, leading to a decrease in fluorescence intensity in the presence of NMM due to the difficulty in G-quadruplex formation. Thus, KF- activity was sensitively detected using NMM. In addition, daidzein, the major phytoestrogen in soy, inhibited DNA synthesis mediated by KF-, and the half maximal inhibitory concentration (IC50) was determined to be 15.6 mu M by nonlinear regression analysis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.relation.isPartOf | ANALYTICAL METHODS | - |
dc.title | Label-free simple fluorescence assay of DNA polymerase using the G-quadruplex structure | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000367243600007 | - |
dc.identifier.doi | 10.1039/c5ay02379c | - |
dc.identifier.bibliographicCitation | ANALYTICAL METHODS, v.8, no.2, pp.275 - 279 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84952941170 | - |
dc.citation.endPage | 279 | - |
dc.citation.startPage | 275 | - |
dc.citation.title | ANALYTICAL METHODS | - |
dc.citation.volume | 8 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Kim, Seyeon | - |
dc.contributor.affiliatedAuthor | Gang, Jongback | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | BREAST-CANCER CELLS | - |
dc.subject.keywordPlus | SWITCH-ON DETECTION | - |
dc.subject.keywordPlus | PEROXIDASE-ACTIVITY | - |
dc.subject.keywordPlus | GENISTEIN | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | PHYTOESTROGENS | - |
dc.subject.keywordPlus | STRATEGY | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | PROBE | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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