The Regulatory Role of Rolipram on Inflammatory Mediators and Cholinergic/Adrenergic Stimulation-Induced Signals in Isolated Primary Mouse Submandibular Gland Cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Dong Un | - |
dc.contributor.author | Shin, Dong Min | - |
dc.contributor.author | Hong, Jeong Hee | - |
dc.date.available | 2020-02-28T06:43:40Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0962-9351 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9715 | - |
dc.description.abstract | Exposure to bacterial lipopolysaccharides (LPS) induces inflammatory signals in salivary glands. We investigated the regulatory role of phosphodiesterase 4 (PDE4) inhibitor rolipram on inflammatory mediators and cholinergic/adrenergic stimulation-induced intracellular Ca2+ signaling in salivary acinar and ductal cells. Submandibular gland (SMG) expressed PDE4A through 4D mRNA and PDE4 was localized in the luminal membrane of SMG. LPS induced Ca2+ signaling and ROS production in SMG. Treatment with rolipram blocked LPS-induced Ca2+ increase and ROS production. The application of histamine evoked Ca2+ signals and ROS production, which were attenuated by rolipram in SMG cells. Moreover, LPS-induced NLRP3 inflammasome and cleaved caspase-1 were inhibited by rolipram. The inhibitory role of rolipram in ROS-induced Ca2+ signaling was mainly observed in acinar cells and not in ductal cells. Rolipram also protected SMG acinar but not ductal cells from LPS-induced cell membrane damage. In the case of cholinergic/adrenergic stimulation, carbachol/isoproterenol-induced Ca2+ signals were upregulated by the treatment of rolipram in SMG. In the case of cAMP-dependent ductal bicarbonate secretion by rolipram, no effect was observed on the modulation of ductal chloride/bicarbonate exchange activity. Rolipram could suppress the inflammatory signals and could be a potential therapeutic strategy against LPS-induced inflammation to protect the salivary gland cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | HINDAWI LTD | - |
dc.relation.isPartOf | MEDIATORS OF INFLAMMATION | - |
dc.subject | TOLL-LIKE RECEPTOR | - |
dc.subject | SALIVARY-GLAND | - |
dc.subject | CALCIUM MOBILIZATION | - |
dc.subject | EXPRESSION | - |
dc.subject | INHIBITOR | - |
dc.subject | PHOSPHODIESTERASES | - |
dc.subject | INTERLEUKIN-8 | - |
dc.subject | IRRADIATION | - |
dc.subject | SECRETION | - |
dc.subject | TLR4 | - |
dc.title | The Regulatory Role of Rolipram on Inflammatory Mediators and Cholinergic/Adrenergic Stimulation-Induced Signals in Isolated Primary Mouse Submandibular Gland Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000374423400001 | - |
dc.identifier.doi | 10.1155/2016/3745961 | - |
dc.identifier.bibliographicCitation | MEDIATORS OF INFLAMMATION | - |
dc.identifier.scopusid | 2-s2.0-84965125120 | - |
dc.citation.title | MEDIATORS OF INFLAMMATION | - |
dc.contributor.affiliatedAuthor | Lee, Dong Un | - |
dc.contributor.affiliatedAuthor | Hong, Jeong Hee | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TOLL-LIKE RECEPTOR | - |
dc.subject.keywordPlus | SALIVARY-GLAND | - |
dc.subject.keywordPlus | CALCIUM MOBILIZATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | PHOSPHODIESTERASES | - |
dc.subject.keywordPlus | INTERLEUKIN-8 | - |
dc.subject.keywordPlus | IRRADIATION | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | TLR4 | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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