Effect of stromal cell derived factor-1 alpha release from heparin-coated Co-Cr stent substrate on the recruitment of endothelial progenitor cells
DC Field | Value | Language |
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dc.contributor.author | Kang, Sung Nam | - |
dc.contributor.author | Park, Chungwon | - |
dc.contributor.author | Kim, Seong Min | - |
dc.contributor.author | Park, Ki Wan | - |
dc.contributor.author | Park, Bang Ju | - |
dc.contributor.author | Han, Dong Keun | - |
dc.contributor.author | Joung, Yoon Ki | - |
dc.date.available | 2020-02-28T07:41:27Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-12 | - |
dc.identifier.issn | 1598-5032 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9842 | - |
dc.description.abstract | The restoration of a damaged endothelium might be a fascinating way to reduce significantly late-thrombosis and restenosis in the stent treatment. It has been recently reported that the recruitment of endothelial progenitor cells (EPCs), capable for repairing a damaged endothelium, can be important in the vascular stent application. Therefore, we focused on the hypothesis that stromal cell-derived factor-1 alpha (SDF-1 alpha) acts as a key chemokine for the mobilization and recruitment of EPCs to the damaged endothelium lesion. In this study, the effect of SDF-1 alpha released from a cobalt-chromium alloy (Co-Cr) plate, vascular stent material, was investigated on the recruitment of EPCs in vitro. Dopamine-conjugated heparin was synthesized to introduce heparin onto Co-Cr. Heparin-coated Co-Cr surfaces were examined by water contact angle and attenuated total reflectance-Fourier transform infrared (ATRFTIR) to prove successful coating of heparin. Finally, SDF-1 alpha was bound to the coated heparin derivative. Amounts of loaded and released SDF-1 alpha were measured by ELISA, and the recruited EPC by released SDF-1 alpha was evaluated using two different migration assays. The quantity of SDF-1 alpha bound to the heparin-modified surface increased in a concentration-dependent manner and SDF-1 alpha was released over 28 days. Transwell migration assay revealed that soluble SDF-1 alpha released from the heparin-coated Co-Cr induced significantly more EPC recruitment than bare Co-Cr and heparin-coated Co-Cr. More importantly, fibrin gel migration assay further demonstrated that EPCs evidently respond to heparin-based substrate with SDF-1 alpha and this type of behaviors was surprisingly found at as low level as less 1 ng/day of SDF-1 alpha. These results are strongly encouraging for further in vitro and in vivo studies. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.relation.isPartOf | MACROMOLECULAR RESEARCH | - |
dc.subject | DRUG-ELUTING STENTS | - |
dc.subject | RE-ENDOTHELIALIZATION | - |
dc.subject | VASCULAR GRAFTS | - |
dc.subject | IN-VITRO | - |
dc.subject | HYDROGELS | - |
dc.subject | SURFACE | - |
dc.subject | BIOCOMPATIBILITY | - |
dc.subject | ENHANCEMENT | - |
dc.subject | SDF-1-ALPHA | - |
dc.subject | CONJUGATE | - |
dc.title | Effect of stromal cell derived factor-1 alpha release from heparin-coated Co-Cr stent substrate on the recruitment of endothelial progenitor cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000366626300012 | - |
dc.identifier.doi | 10.1007/s13233-015-4002-z | - |
dc.identifier.bibliographicCitation | MACROMOLECULAR RESEARCH, v.23, no.12, pp.1159 - 1167 | - |
dc.identifier.kciid | ART002063809 | - |
dc.identifier.scopusid | 2-s2.0-84949955668 | - |
dc.citation.endPage | 1167 | - |
dc.citation.startPage | 1159 | - |
dc.citation.title | MACROMOLECULAR RESEARCH | - |
dc.citation.volume | 23 | - |
dc.citation.number | 12 | - |
dc.contributor.affiliatedAuthor | Park, Bang Ju | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | stent | - |
dc.subject.keywordAuthor | dopamine | - |
dc.subject.keywordAuthor | heparin | - |
dc.subject.keywordAuthor | stromal cell-derived factor-1 alpha | - |
dc.subject.keywordAuthor | endothelial progenitor cell | - |
dc.subject.keywordAuthor | recruitment | - |
dc.subject.keywordPlus | DRUG-ELUTING STENTS | - |
dc.subject.keywordPlus | RE-ENDOTHELIALIZATION | - |
dc.subject.keywordPlus | VASCULAR GRAFTS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | HYDROGELS | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | BIOCOMPATIBILITY | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordPlus | SDF-1-ALPHA | - |
dc.subject.keywordPlus | CONJUGATE | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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