AKT is translocated to the mitochondria during etoposide-induced apoptosis of HeLa cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Byoungduck | - |
dc.contributor.author | Je, Young-Tae | - |
dc.contributor.author | Chun, Kwang-Hoon | - |
dc.date.available | 2020-02-28T07:43:23Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-11 | - |
dc.identifier.issn | 1791-2997 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9953 | - |
dc.description.abstract | Akt, or protein kinase B, is a key serine-threonine kinase, which exerts anti-apoptotic effects and promotes cell proliferation in response to various stimuli. Recently, however, it was demonstrated that Akt exhibits a proapoptotic role in certain contexts. During etoposide-induced apoptosis of He La cells, Akt enhances the interaction of second mitochondria-derived activator of caspases/direct IAP binding protein with low pI (Smac/DIABLO) and X-linked inhibitor of apoptosis protein by phosphorylating Smac at serine 67, and thus promotes apoptosis. However, the detailed mechanisms underlying Akt regulation in etoposide-mediated apoptosis remain to be determined. The present study investigated whether etoposide triggers the translocation of Akt into the mitochondria. It was found that Akt activity was increased and sustained during apoptosis triggered by etoposide in He La cells. During apoptosis, Akt was translocated from the cytoplasm into the mitochondria in a phosphoinositide 3-kinase-dependent manner at the early and late stages of apoptosis. Concomitantly, the depletion of Akt in the nuclear fraction was observed after etoposide treatment from analysis of confocal microscopy. The results suggest that etoposide-stimulated Akt is translocated into the mitochondria, thereby possibly enhancing its interaction with Smac and promoting apoptosis in He La cells. These results indicate that Akt may be a promising candidate for a pro-apoptotic approach in cancer treatment. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.relation.isPartOf | MOLECULAR MEDICINE REPORTS | - |
dc.subject | PROTEIN-KINASE-B | - |
dc.subject | TYROSINE KINASE | - |
dc.subject | MEDIATED APOPTOSIS | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | CYTOCHROME-C | - |
dc.subject | DEATH | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | ACTIVATION | - |
dc.subject | PATHWAY | - |
dc.subject | SURVIVAL | - |
dc.title | AKT is translocated to the mitochondria during etoposide-induced apoptosis of HeLa cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000364889100050 | - |
dc.identifier.doi | 10.3892/mmr.2015.4378 | - |
dc.identifier.bibliographicCitation | MOLECULAR MEDICINE REPORTS, v.12, no.5, pp.7577 - 7581 | - |
dc.identifier.scopusid | 2-s2.0-84943623827 | - |
dc.citation.endPage | 7581 | - |
dc.citation.startPage | 7577 | - |
dc.citation.title | MOLECULAR MEDICINE REPORTS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | Chun, Kwang-Hoon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Akt | - |
dc.subject.keywordAuthor | mitochondria | - |
dc.subject.keywordAuthor | translocation | - |
dc.subject.keywordPlus | PROTEIN-KINASE-B | - |
dc.subject.keywordPlus | TYROSINE KINASE | - |
dc.subject.keywordPlus | MEDIATED APOPTOSIS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | CYTOCHROME-C | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.