Betacellulin ameliorates hyperglycemia in obese diabetic db/db mice
DC Field | Value | Language |
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dc.contributor.author | Oh, Yoon Sin | - |
dc.contributor.author | Shin, Seungjin | - |
dc.contributor.author | Li, Hui Ying | - |
dc.contributor.author | Park, Eun-Young | - |
dc.contributor.author | Lee, Song Mi | - |
dc.contributor.author | Choi, Cheol Soo | - |
dc.contributor.author | Lim, Yong | - |
dc.contributor.author | Jung, Hye Seung | - |
dc.contributor.author | Jun, Hee-Sook | - |
dc.date.available | 2020-02-28T07:43:31Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-11 | - |
dc.identifier.issn | 0946-2716 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/9963 | - |
dc.description.abstract | We found that administration of a recombinant adenovirus (rAd) expressing betacellulin (BTC) into obese diabetic db/db mice ameliorated hyperglycemia. Exogenous glucose clearance was significantly improved, and serum insulin levels were significantly higher in rAd-BTC-treated mice than rAd-beta-gal-treated control mice. rAd-BTC treatment increased insulin/bromodeoxyuridine double-positive cells in the islets, and islets from rAd-BTC-treated mice exhibited a significant increase in the level of G1-S phase-related cyclins as compared with control mice. In addition, BTC treatment increased messenger RNA (mRNA) and protein levels of these cyclins and cyclin-dependent kinases in MIN-6 cells. BTC treatment induced intracellular Ca2+ levels through phospholipase C-gamma 1 activation, and upregulated calcineurin B (CnB1) levels as well as calcineurin activity. Upregulation of CnB1 by BTC treatment was observed in isolated islet cells from db/db mice. When treated with CnB1 small interfering RNA (siRNA) in MIN-6 cells and isolated islets, induction of cell cycle regulators by BTC treatment was blocked and consequently reduced BTC-induced cell viability. As well as BTC's effects on cell survival and insulin secretion, our findings demonstrate a novel pathway by which BTC controls beta-cell regeneration in the obese diabetic condition by regulating G1-S phase cell cycle expression through Ca2+ signaling pathways. Key messages Administration of BTC to db/db mice results in amelioration of hyperglycemia. BTC stimulates beta-cell proliferation in db/db mice. Ca2+ signaling was involved in BTC-induced beta-cell proliferation. BTC has an anti-apoptotic effect and potentiates glucose-stimulated insulin secretion. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.relation.isPartOf | JOURNAL OF MOLECULAR MEDICINE-JMM | - |
dc.subject | PANCREATIC BETA-CELL | - |
dc.subject | GROWTH | - |
dc.subject | INSULIN | - |
dc.subject | PROLIFERATION | - |
dc.subject | STIMULATION | - |
dc.subject | PATHWAYS | - |
dc.subject | RECEPTOR | - |
dc.subject | GLUCOSE | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | REGENERATION | - |
dc.title | Betacellulin ameliorates hyperglycemia in obese diabetic db/db mice | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000365715100008 | - |
dc.identifier.doi | 10.1007/s00109-015-1303-1 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MOLECULAR MEDICINE-JMM, v.93, no.11, pp.1235 - 1245 | - |
dc.identifier.scopusid | 2-s2.0-84946493834 | - |
dc.citation.endPage | 1245 | - |
dc.citation.startPage | 1235 | - |
dc.citation.title | JOURNAL OF MOLECULAR MEDICINE-JMM | - |
dc.citation.volume | 93 | - |
dc.citation.number | 11 | - |
dc.contributor.affiliatedAuthor | Oh, Yoon Sin | - |
dc.contributor.affiliatedAuthor | Li, Hui Ying | - |
dc.contributor.affiliatedAuthor | Lee, Song Mi | - |
dc.contributor.affiliatedAuthor | Choi, Cheol Soo | - |
dc.contributor.affiliatedAuthor | Jun, Hee-Sook | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Betacellulin | - |
dc.subject.keywordAuthor | db/db mice | - |
dc.subject.keywordAuthor | Cellcycle | - |
dc.subject.keywordAuthor | Calcineurin B1 | - |
dc.subject.keywordAuthor | Beta cell regeneration | - |
dc.subject.keywordPlus | PANCREATIC BETA-CELL | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | INSULIN | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | STIMULATION | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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