https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/377 2026-07-04T15:46:16Z 2026-07-04T15:46:16Z 한상윤 https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/217571 2026-07-02T18:34:30Z 2026-06-14T00:00:00Z

Title: 메니에르병: current approach and treatment Authors: 한상윤

2026-06-14T00:00:00Z 송창면 https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/217771 2026-07-01T23:33:40Z 2026-06-13T00:00:00Z

Title: IONM Update and New Clinical Applications Authors: 송창면

2026-06-13T00:00:00Z Han, Sang-Yoon Park, Jae Keun Choi, Ji Suk Jeong, Eun Ji Eom, Min Rye Seok, Ji Min Kim, Min Ji Park, Su A. Oh, Se Heang Kwon, Seong Keun https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/211011 2026-03-03T05:00:54Z 2026-06-01T00:00:00Z

Title: Enhanced MSC spheroid adhesion on 3D-printed leaf-stacked scaffolds for functional tracheal regeneration Authors: Han, Sang-Yoon; Park, Jae Keun; Choi, Ji Suk; Jeong, Eun Ji; Eom, Min Rye; Seok, Ji Min; Kim, Min Ji; Park, Su A.; Oh, Se Heang; Kwon, Seong Keun Abstract: Reconstruction of segmental tracheal defects using three-dimensional (3D)-printed scaffolds remains a formidable challenge. While polycaprolactone (PCL) is widely utilized for its mechanical integrity, its inherent hydrophobicity limits cellular adhesion and tissue integration. In this study, we developed a 3D-printed PCL tracheal scaffold featuring a Leaf-Stacked Structure (LSS) and evaluated a spatially organized Mesenchymal Stem Cell (MSC) delivery strategy for functional regeneration. MSC spheroids were employed to overcome the limitations of monolayer cells, as their 3D configuration creates an internal hypoxic core that upregulates angiogenic and anti-inflammatory genes, thereby maximizing paracrine-mediated tissue repair. In vitro analyses, including cell adhesion assays and indirect co-culture systems, demonstrated that the LSS topography significantly enhanced the adhesion of both monolayer MSC and spheroids compared to plain PCL. Furthermore, MSC spheroids markedly promoted the proliferation and migration of human small airway epithelial cells. Based on these findings, we compared five experimental groups in a rabbit tracheal defect model: (1) Native, (2) No MSC, (3) Inner MSC (monolayer), (4) Outer spheroid, and (5) Dual group (combined inner monolayer and outer spheroids). In vivo, the Dual group exhibited the most robust mucosal regeneration, alongside an immunomodulatory shift toward increased M2/M1 macrophage ratios. Although neovascularization was prominent at MSC implantation sites, lineage analysis via β2-microglobulin tracking revealed that vessel-forming cells were primarily host-derived. This confirms that implanted MSC survived for 14 weeks and orchestrated regeneration predominantly through paracrine mechanisms.Collectively, the integration of LSS topography and spatially organized MSC represents a promising synergistic strategy for functional tracheal reconstruction.

2026-06-01T00:00:00Z Han, Sang-Yoon Suh, Myung-Whan Park, Moo Kyun Lee, Jun Ho Kang, Hee Gyung Lee, Sang-Yeon https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/212773 2026-05-20T06:00:08Z 2026-05-01T00:00:00Z

Title: Hearing loss phenotypes in Alport syndrome: experience in a tertiary referral center Authors: Han, Sang-Yoon; Suh, Myung-Whan; Park, Moo Kyun; Lee, Jun Ho; Kang, Hee Gyung; Lee, Sang-Yeon Abstract: Background: Despite previous reports of auditory phenotypes in Alport syndrome (AS), there have been no studies specifically addressing audiological phenotypes in South Korea. Herein, we elaborated on the audiological characteristics associated with AS based on their genotypes. Methods: We reviewed data from in-house AS patients between March 2014 and February 2023, excluding those without audiological documentation or genetic diagnoses. We retrieved medical history, hearing level, estimated glomerular filtration rate (eGFR), and genotypes from their medical records. The natural course of hearing loss and correlations between audiogram and eGFR were evaluated according to audio-gene profiles. Results: Our study included 49 AS patients from 47 families, identifying 60 disease-causing variants, 45 of which were novel. All variants were classified as pathogenic or likely pathogenic based on ACMG-AMP guidelines. The auditory phenotypes of autosomal recessive AS (ARAS) and male X-linked AS (XLAS) patients demonstrated a progressive nature, with a down-sloping configuration. The ARAS with truncated variants exhibited an earlier onset of hearing loss than those with non-truncated variants. In male XLAS patients, the presence of truncated allele linked to more rapid hearing deterioration across all frequencies. In both ARAS and male XLAS patients, the presence of truncated allele was significantly associated with hearing severity and eGFR. Conversely, the majority of female XLAS and autosomal dominant AS maintained normal hearing levels without any correlation of eGFR, regardless of genotypes. Conclusion: This study detailed the auditory phenotypes and the auditory-renal association of AS at a tertiary center in South Korea, providing valuable references that guide auditory testing and rehabilitation strategies.

2026-05-01T00:00:00Z