https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/378 2026-07-04T09:30:42Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/211011 Title: Enhanced MSC spheroid adhesion on 3D-printed leaf-stacked scaffolds for functional tracheal regeneration Authors: Han, Sang-Yoon; Park, Jae Keun; Choi, Ji Suk; Jeong, Eun Ji; Eom, Min Rye; Seok, Ji Min; Kim, Min Ji; Park, Su A.; Oh, Se Heang; Kwon, Seong Keun Abstract: Reconstruction of segmental tracheal defects using three-dimensional (3D)-printed scaffolds remains a formidable challenge. While polycaprolactone (PCL) is widely utilized for its mechanical integrity, its inherent hydrophobicity limits cellular adhesion and tissue integration. In this study, we developed a 3D-printed PCL tracheal scaffold featuring a Leaf-Stacked Structure (LSS) and evaluated a spatially organized Mesenchymal Stem Cell (MSC) delivery strategy for functional regeneration. MSC spheroids were employed to overcome the limitations of monolayer cells, as their 3D configuration creates an internal hypoxic core that upregulates angiogenic and anti-inflammatory genes, thereby maximizing paracrine-mediated tissue repair. In vitro analyses, including cell adhesion assays and indirect co-culture systems, demonstrated that the LSS topography significantly enhanced the adhesion of both monolayer MSC and spheroids compared to plain PCL. Furthermore, MSC spheroids markedly promoted the proliferation and migration of human small airway epithelial cells. Based on these findings, we compared five experimental groups in a rabbit tracheal defect model: (1) Native, (2) No MSC, (3) Inner MSC (monolayer), (4) Outer spheroid, and (5) Dual group (combined inner monolayer and outer spheroids). In vivo, the Dual group exhibited the most robust mucosal regeneration, alongside an immunomodulatory shift toward increased M2/M1 macrophage ratios. Although neovascularization was prominent at MSC implantation sites, lineage analysis via β2-microglobulin tracking revealed that vessel-forming cells were primarily host-derived. This confirms that implanted MSC survived for 14 weeks and orchestrated regeneration predominantly through paracrine mechanisms.Collectively, the integration of LSS topography and spatially organized MSC represents a promising synergistic strategy for functional tracheal reconstruction. 2026-06-01T00:00:00Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/212773 Title: Hearing loss phenotypes in Alport syndrome: experience in a tertiary referral center Authors: Han, Sang-Yoon; Suh, Myung-Whan; Park, Moo Kyun; Lee, Jun Ho; Kang, Hee Gyung; Lee, Sang-Yeon Abstract: Background: Despite previous reports of auditory phenotypes in Alport syndrome (AS), there have been no studies specifically addressing audiological phenotypes in South Korea. Herein, we elaborated on the audiological characteristics associated with AS based on their genotypes. Methods: We reviewed data from in-house AS patients between March 2014 and February 2023, excluding those without audiological documentation or genetic diagnoses. We retrieved medical history, hearing level, estimated glomerular filtration rate (eGFR), and genotypes from their medical records. The natural course of hearing loss and correlations between audiogram and eGFR were evaluated according to audio-gene profiles. Results: Our study included 49 AS patients from 47 families, identifying 60 disease-causing variants, 45 of which were novel. All variants were classified as pathogenic or likely pathogenic based on ACMG-AMP guidelines. The auditory phenotypes of autosomal recessive AS (ARAS) and male X-linked AS (XLAS) patients demonstrated a progressive nature, with a down-sloping configuration. The ARAS with truncated variants exhibited an earlier onset of hearing loss than those with non-truncated variants. In male XLAS patients, the presence of truncated allele linked to more rapid hearing deterioration across all frequencies. In both ARAS and male XLAS patients, the presence of truncated allele was significantly associated with hearing severity and eGFR. Conversely, the majority of female XLAS and autosomal dominant AS maintained normal hearing levels without any correlation of eGFR, regardless of genotypes. Conclusion: This study detailed the auditory phenotypes and the auditory-renal association of AS at a tertiary center in South Korea, providing valuable references that guide auditory testing and rehabilitation strategies. 2026-05-01T00:00:00Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/214344 Title: Postoperative pain outcomes following thyroidectomy: a systematic review and network meta-analysis of various surgical approaches Authors: Nguyen, Van Cuong; Song, Chang Myeon; Ji, Yong Bae; Moon, Shinje; Park, Jung Hwan; Russell, Jonathon O.; Randolph, Gregory W.; Tae, Kyung Abstract: Purpose: Remote-access thyroidectomy techniques and minimally invasive video-assisted thyroidectomy (MIVAT) have been developed to optimize cosmetic outcomes and potentially improve recovery, particularly in the case of MIVAT, compared with the conventional transcervical approach (CTA). However, comparative evidence regarding postoperative pain outcomes remains limited. This study aims to evaluate postoperative pain outcomes among MIVAT and five remote-access approaches-breast approach (BA), bilateral axillo-breast approach (BABA), gasless transaxillary approach (GTAA), retroauricular approach (RA), and transoral approach (TOA)-in comparison with CTA. Methods: A systematic review and network meta-analysis were conducted. PUBMED, EMBASE, and the Cochrane Library were searched through March 2025. Pain outcomes across surgical approaches were compared using a frequentist random-effects network meta-analysis. Results: Sixty-one studies involving 9,780 patients were included. On postoperative day one, MIVAT was associated with the lowest pain scores, followed by the BA and TOA. At one-week and one-month follow-ups, MIVAT and TOA consistently exhibited the most favorable pain outcomes. Conversely, the RA and BABA were linked to the highest pain scores on day one and at one week. Differences in postoperative pain between robotic and endoscopic techniques within the same approach were not significant, although robotic RA and robotic TOA showed lower pain scores than those of their endoscopic counterparts. Conclusion: MIVAT and TOA provide superior postoperative pain outcomes than those of CTA and other remote-access approaches. Robotic techniques may offer additional benefits over endoscopic methods in selected cases. These findings may support more patient-centered surgical decision-making. 2026-05-01T00:00:00Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/217609 Title: A Content Analysis of YouTube Videos on Facial Palsy in South Korea Authors: Hong, Seong Man; Kim, So Yeon; Han, Sang-Yoon; Seo, Hee Won; Chung, Jae Ho; Lee, Seung Hwan Abstract: Background and Objectives YouTube has become a major source of health information,raising concerns regarding the accuracy of medical content. Facial nerve paralysis is a clinically significant condition with substantial functional, social, emotional, and economic impact. However, only limited studies have evaluated the quality of YouTube videos addressingfacial palsy. This study aimed to review video content and assess the quality of informationrelated to facial palsy on YouTube. Subjects and Method A YouTube search was performed using five Korean keywords: “안면신경마비,” “벨마비,” “람세이헌트,” “얼굴마비,” and “구안와사.” The top 100 Korean-language videos were screened using a newly created account. Videos unrelated to facial palsywere excluded. Content creators were categorized by professional background. Video qualitywas assessed using the Patient Education Materials Assessment Tool for Audiovisual Materials and a modified DISCERN score. Results Among the 100 included videos, 9 were produced by otolaryngologists, 44 by traditional Korean medicine doctors, 27 by other medical professionals, and 20 by lay persons. Bell’s palsy was the most commonly discussed condition, whereas Ramsay Hunt syndrome,central facial palsy, and traumatic facial paralysis were less frequently addressed. Otolaryngologists’ videos covered verified treatments such as corticosteroids, antiviral drugs, and protective agents for the eyes. Otolaryngologists’ videos showed significantly higher understandability and quality of information compared to others. Conclusion YouTube videos on facial palsy frequently contain low-quality or unverified information, which may negatively influence patients. The findings in this study highlight theimportance of specialist involvement in providing accurate and reliable medical information. 2026-05-01T00:00:00Z