https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/450 2026-07-04T02:02:18Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/212896 Title: Functional impact of the ATP1A3-p.A813V variant: insights into a calcium-driven hyperexcitability cascade in rapid-onset dystonia-Parkinsonism Authors: Lim, Su Min; Kim, Suhyun; Park, Jinseok; Kim, Young-Eun; Na, Ok Cho; Nahm, Minyeop; Noh, Min-Young; Oh, Ki-Wook; Ki, Chang-Seok; Shin, Woong-Hee; Park, Hae-Chul; Kim, Seung Hyun Abstract: Background Mutations in the neuronal Na+/K+-ATPase subunit ATP1A3 are linked to a spectrum of neurological disorders, including rapid-onset dystonia-parkinsonism (RDP), yet their pathogenic mechanisms remain incompletely understood. We describe the complex clinical characteristics of a patient with early-onset movement disorders and a likely pathogenic de novo variant in ATP1A3(c & centerdot;2438C>T, p.A813V). Methods We identified a de novo heterozygous ATP1A3 p.A813V variant in a patient with clinically confirmed RDP and employed an integrative approach combining molecular dynamics (MD) simulations, zebrafish models, and patient-derived induced neurons (iNeurons) to delineate its pathogenesis. Results MD simulations revealed that the p.A813V substitution structurally distorts transmembrane helix packing, reduces structural stability, and diminishes water accessibility at the cation-binding site, predicting impaired Na+/K+-ATPase function. In vivo, atp1a3b knockout zebrafish developed pronounced neuronal hyperexcitability-reflected by elevated c-fos and pERK expression-that emerged before overt neurodegeneration, motor axonopathy, and neuromuscular junction defects. Complementarily, neurons expressing ATP1A3-p.A813V displayed significantly prolonged calcium transient decay times, suggesting a potential mechanism of altered Ca2+ handling and delayed clearance mechanisms compatible with ATP1A3 dysfunction. Consistent with these findings, patient-derived iNeurons exhibited markedly reduced ATP1A3 protein abundance and Na+/K+-ATPase activity. Conclusions Together, these findings lead us to propose a mechanistic model in which ATP1A3 dysfunction disrupts Ca2+ homeostasis, triggers neuronal hyperexcitability, and culminates in progressive neurodegeneration. This work provides a molecular and functional framework for targeting ionic and calcium homeostasis as a promising therapeutic strategy for ATP1A3-related disorders. 2026-05-01T00:00:00Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/217619 Title: Molecular and Microbiological Characteristics of Uropathogenic Escherichia coli Harboring CTX-M-55 Authors: Kwon, Heejeong; Kim, Jeoungyeon; Kim, Jinnam; Park, Se Yoon; Yoon, Choseok; Lee, Yangsoon; Kim, Bongyoung Abstract: The extended-spectrum β-lactamase (ESBL) CTX-M-55, a CTX-M-15 variant distinguished by an amino-acid substitution (Ala77Val), has enhanced enzymatic activity due to higher structural stability. In Korea, CTX-M-55 remains insufficiently characterized, particularly in the context of urinary tract infections (UTIs). We identified CTX-M-55 among uropathogenic Escherichia coli isolates and compared its microbiological characteristics with those of CTX-M-15. In total, 247 E. coli isolates were collected from patients with acute pyelonephritis at Hanyang University Seoul Hospital, an 860-bed tertiary-care hospital, between July 2019 and December 2021. ESBL production was confirmed using a double-disk synergy test, and minimum inhibitory concentrations (MICs) were determined. Resistance genes were detected using PCR, and CTX-M-15 sequences were analyzed. Among 38 isolates detected using PCR, eight were confirmed as CTX-M-55 using further sequence analysis. CTX-M-55 showed (P >0.05) a trend toward increased resistance to aztreonam, cefotaxime, ceftazidime, and cefepime, while showing decreased resistance to amoxicillin/clavulanate and piperacillin/tazobactam. CTX-M-55 had higher MIC50 values than CTX-M-15 for ceftazidime (>16 vs. 8 µg/mL), cefepime (32 vs. 1 µg/mL), and piperacillin/tazobactam (0.5 vs. 0.25 µg/mL). Virulence factors and coexisting resistance genes did not significantly differ. Our findings suggest that, given its increased resistance to ceftazidime and cefepime, CTX-M-55 should be considered when treating UTIs in Korea. 2026-05-01T00:00:00Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/217704 Title: Comparison of the effectiveness of automatic and manual plasma-treated hydrogen peroxide mist disinfection in various teaching hospital environments Authors: Choi, Jongbong; Lee, Yangsoon; Kim, Yunjin; Lim, Tae Ho Abstract: Background Automatic disinfection technologies have been developed to improve the reliability and thoroughness of hospital disinfection. However, it is not clear whether automated systems can achieve similar disinfection results to those obtained by well-trained professionals using manual methods. We evaluated the disinfection efficacies of automatic and manual plasma-treated hydrogen peroxide mist (PTHPM) systems in various hospital environments. Methods Disinfection was performed in 23 rooms in a teaching hospital, covering various hospital wards, outpatient departments, and emergency rooms. Overall, 459 surfaces were swabbed before and after disinfection. Only gram-positive bacteria were analyzed statistically owing to the low prevalence of gram-negative bacteria and molds. Results Before disinfection, the viability of gram-positive bacteria, based on colony-forming units, was highest in outpatient departments, followed by emergency rooms and hospital wards using both automatic and manual disinfection. Automatic PTHPM disinfection reduced the colony-forming units of gram-positive bacteria significantly in various environments. There were no significant differences in the effectiveness of automated and manual PTHPM disinfection. Conclusions Automated PTHPM disinfection can be as effective as manual PTHPM disinfection in eliminating microbial contamination in teaching hospital environments. 2026-05-01T00:00:00Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/217700 Title: Nanofibrous Interface-Engineered Microgel Array Enabling Streamlined Digital PCR Authors: Kim, Serin; Jang, Yoon-ha; Lee, Sang-A; Jeon, Eui Ju; Choi, Song-Ee; Kim, Tae Jong; Chung, Seok; Park, Kuenyoul; Sung, Heungsup; Jung, Seungwon; Kim, Sang Kyung Abstract: Digital polymerase chain reaction (dPCR) is increasingly used due to its key advantageshigh sensitivity, absolute quantification, and tolerance to contaminantsthat contribute to its overall robustness. However, it typically relies on complex droplet or microfluidic systems requiring pumps or vacuum systems for partitioning, limiting its accessibility and portability. Here, we present a microgel array chip comprising 2003 porous microgels (1 nL) in a 1 cm2 area, offering simple and rapid partitioning. Controlling internal porosity and surface structure of microgels is crucial to achieving reliable volume control, effective mass transfer, and amplification. In particular, the hydrophilic, oil-repelling nanofibrous architecture of the microgel surface enables reliable partitioning via sequential injection of the PCR mixture and oil. Its solid structure also provides stable amplification under fast thermal cycling, allowing the diagnostic assay to be completed in under 1 h. The platform demonstrates high accuracy in absolute quantification of nucleic acids, showing a strong correlation with conventional RT-qPCR in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nasopharyngeal swab samples. These results highlight the microgel array dPCR as a practical and accessible tool for rapid and accurate diagnostics. 2026-04-01T00:00:00Z