https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/347 Sat, 04 Jul 2026 05:58:02 GMT 2026-07-04T05:58:02Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/213857 Title: Treatment nonresponders in lupus nephritis characteristically exhibit persistent type I interferon signalling in monocytes: a longitudinal single-cell transcriptomic analysis Authors: Kim, Woo-Joong; Lee, Hye-Soon; Bang, So-Young; Bin Joo, Young; Kang, Bo-Kyeong; Kim, Mimi; Kim, Hyunsung; Park, Sung Yul; Park, Woong-Yang; Shin, Eui-Cheol; Bae, Sang-Cheol Abstract: Objectives: We aimed to characterise the dynamic transcriptional changes associated with treatment responses in lupus nephritis (LN) through longitudinal single-cell RNA sequencing analysis of peripheral blood mononuclear cells (PBMCs) during standard-of-care induction therapy. Methods: We leveraged the Korean Unlimited multi-Dimensional Omics research in Systemic lupus erythematosus cohort, comprising patients with biopsy-proven active proliferative LN. Single-cell RNA sequencing of PBMCs was conducted at baseline and subsequently at 3, 6, and 12 months following initiation of therapy (n = 10). For validation purposes, bulk RNA sequencing of monocytes was performed in an independent patient group at baseline and at 3 months (n = 13). Renal response was classified as complete response or nonresponse at 12 months according to predefined criteria. Results: In single-cell RNA sequencing analysis of patients with LN, the myeloid cell population—particularly classical and intermediate monocytes—demonstrated the highest number of differentially expressed genes following induction therapy. Weighted gene coexpression network analysis identified distinct gene modules closely associated with treatment responses. Complete responders exhibited progressive suppression of type I interferon (IFN-I) signalling, whereas nonresponders maintained persistent IFN-I-driven gene expression characterised by sustained inflammatory features. Bulk RNA sequencing of monocytes from an independent group of patients with LN confirmed that 6 IFN-I-responsive genes (IRF7, ISG15, LY6E, IFI44, IFI44L, and IFI6) were significantly downregulated by 3 months in complete responders, but not in nonresponders. This gene signature correlated with both proteinuria and disease activity scores. Conclusions: This study demonstrates that persistent IFN-I-driven gene expression in monocytes characterises treatment resistance in LN. Our findings suggest that early transcriptional profiling may enable timely identification of nonresponders and warrant further investigation in larger, independent cohorts. Wed, 01 Apr 2026 00:00:00 GMT https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/213857 2026-04-01T00:00:00Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/214933 Title: Reassessing age limits: Outcomes of radical nephroureterectomy in octogenarian patients with upper tract urothelial carcinoma Authors: Song, Sang Hun; Lee, Haesung; Chung, Younsoo; Song, Byeongdo; Lee, Hakmin; Oh, Jong Jin; Lee, Sangchul; Byun, Seok-Soo; Hong, Sung Kyu Abstract: Purpose: To assess whether radical nephroureterectomy (RNU) confers oncologic and perioperative benefit in patients aged 80 years or older with upper tract urothelial carcinoma (UTUC). Materials and Methods: This retrospective cohort study included 710 patients who underwent RNU at a tertiary center from 2003 to 2022. Patients were stratified into octogenarian (OG) (>= 80 years, n=96) and non-octogenarian (NOG) (<80 years, n=614) groups. Demographics, perioperative variables, and oncologic outcomes were compared. Multivariate Cox regression was performed for overall survival and cancer-specific survival (CSS). Results: OG had a higher rate of hypertension (72.9% vs. 50.2%) and lower body mass index (23.6 kg/m(2) vs. 24.5 kg/m(2), p=0.028), with similar pathologic stage and tumor grade compared to NOG. OG were more likely to undergo minimally invasive surgery (84.3% vs. 75.3%, p<0.001). Overall complication rate was higher in OG (68.8% vs. 55.2%, p=0.013), but major complications were not (5.2% vs. 3.3%, p=0.340). At median follow-up of 43.6 months, no difference in CSS was found. Positive surgical margin was the only independent predictor of CSS. Conclusions: RNU can be safely and effectively performed in well-selected OG with UTUC. Age >= 80 years was not associated with inferior CSS outcomes or increased major morbidity. Sun, 01 Mar 2026 00:00:00 GMT https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/214933 2026-03-01T00:00:00Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/211324 Title: Cancer-associated fibroblasts are associated with CD8+ T cell depletion and poor prognosis in colorectal adenocarcinoma: a multi-omics and machine learning analysis Authors: Shim, Myungsun; Kim, One-Zoong; Son, Byoung Kwan; Jo, Jung Ki; Lee, Seung Wook; Moon, Hong Sang; Kim, Hyung Suk; Kwon, Mi Jung; Lee, Sung Hak; Noh, Yung-Kyun; Min, Kyueng-Whan Abstract: Fibroblastic proliferation in various tumor microenvironments influences cancer survival through complex interactions with diverse immune responses. This study investigated the impact of histologically unique activated cancer-associated fibroblasts (aCAFs) on survival outcomes and immune responses and examined their association with various pathophysiological mechanisms. We analyzed a total of 1,024 colorectal adenocarcinoma patients from two cohorts. aCAFs were evaluated based on hematoxylin and eosin-stained whole-slide images, and their associations with clinicopathological features, immune cell infiltration, and survival were assessed. We developed a machine learning-based survival prediction model incorporating aCAFs and clinicopathologic parameters. Additionally, we performed differential gene expression analysis, functional enrichment analyses, and in vitro drug screening of aCAF-related genes. aCAFs were associated with advanced T stage, lymphovascular invasion, perineural invasion, and decreased CD8+ and CD4+ T cell infiltration. aCAFs were also associated with worse overall and disease-free survival in both univariate and multivariate analyses. Functional enrichment analysis revealed that aCAF-related genes were implicated in immunosuppressive signaling, oxidative stress regulation, and tumor progression pathways. Survival prediction models based on machine learning and incorporating aCAFs demonstrated superior prognostic accuracy for overall survival and disease-free survival compared to models excluding aCAFs. Our analysis of aCAFs' association with immune responses through bioinformatics-based genomic analysis and machine learning provides a foundation for future research in CRC patients. Sun, 01 Feb 2026 00:00:00 GMT https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/211324 2026-02-01T00:00:00Z https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/210829 Title: Preoperative serum albumin level is associated with postoperative short-and long-term renal function deterioration in patients who underwent radical cystectomy for bladder cancer Authors: Song, Byeongdo; Lee, Hakmin; Lee, Sangchul; Hong, Sung Kyu; Byun, Seok-Soo; Oh, Jong Jin Abstract: Purpose: Radical cystectomy (RC), which is the standard of care for muscle-invasive and high-grade noninvasive bladder cancer, is accompanied by postoperative renal function deterioration. We aimed to evaluate the effect of serum albumin level on postoperative renal function decline after RC. Materials and Methods: A total of 272 patients with estimated glomerular filtration rate (eGFR) >_60 mL/minute/1.73 m(2)who underwent RC between October 2003 and December 2020 were included. Acute kidney injury (AKI) was defined according to the KDIGO (Kidney Disease Improving Global Outcomes) criteria, while postoperative chronic kidney disease (CKD) progression was defined as an eGFR <60 mL/minute/1.73 m(2) at >_3 months after RC. Results: In our cohort, 20 (7.4%) and 99 (36.4%) patients experienced postoperative AKI and CKD progression, respectively, with a median follow-up period of 51.5 months. The median preoperative serum albumin level and eGFR were 4.1 g/dL and 82.0 mL/min-ute/1.73 m(2), respectively. Preoperative serum albumin less than the median (4.1 g/dL) was associated with postoperative AKI (odds ratio [OR] 3.76, p=0.027) and CKD progression (OR 2.87, p<0.001) after adjusting for other factors. Conclusions: Serum albumin level <4.1 g/dL was associated with short-and long-term renal function decline after RC, suggesting that close monitoring of renal function after RC might be considered in these patients. Thu, 01 Jan 2026 00:00:00 GMT https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/210829 2026-01-01T00:00:00Z