Role of MicroRNA-34a in Anti-Apoptotic Effects of Granulocyte-Colony Stimulating Factor in Diabetic Cardiomyopathy
DC Field | Value | Language |
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dc.contributor.author | Park, In-Hwa | - |
dc.contributor.author | Song, Yi-Sun | - |
dc.contributor.author | Joo, Hyun-Woo | - |
dc.contributor.author | Shen, Guang-Yin | - |
dc.contributor.author | Seong, Jin-Hee | - |
dc.contributor.author | Shin, Na-Kyoung | - |
dc.contributor.author | Cho, Young Jong | - |
dc.contributor.author | Lee, Yonggu | - |
dc.contributor.author | Shin, Jeong Hun | - |
dc.contributor.author | Lim, Young-Hyo | - |
dc.contributor.author | Kim, Hyuck | - |
dc.contributor.author | Kim, Kyung-Soo | - |
dc.date.accessioned | 2021-08-02T09:53:57Z | - |
dc.date.available | 2021-08-02T09:53:57Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2020-02 | - |
dc.identifier.issn | 2233-6079 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/10787 | - |
dc.description.abstract | Background: Recent studies have shown that microRNAs (miRNAs) are involved in the process of cardiomyocyte apoptosis. We have previously reported that granulocyte-colony stimulating factor (G-CSF) ameliorated diastolic dysfunction and attenuated cardiomyocyte apoptosis in a rat model of diabetic cardiomyopathy. In this study, we hypothesized a regulatory role of cardiac miRNAs in the mechanism of the anti-apoptotic effect of G-CSF in a diabetic cardiomyopathy rat model. Methods: Rats were given a high-fat diet and low-dose streptozotocin injection and then randomly allocated to receive treatment with either G-CSF or saline. H9c2 rat cardiomyocytes were cultured under a high glucose (HG) condition to induce diabetic cardiomyopathy in vitro. We examined the extent of apoptosis, miRNA expression, and miRNA target genes in the myocardium and H9c2 cells. Results: G-CSF treatment significantly decreased apoptosis and reduced miR-34a expression in diabetic myocardium and H9c2 cells under the HG condition. G-CSF treatment also significantly increased B-cell lymphoma 2 (Bcl-2) protein expression as a target for miR-34a. In addition, transfection with an miR-34a mimic significantly increased apoptosis and decreased Bcl-2 luciferase activity in H9c2 cells. Conclusion: Our results indicate that G-CSF might have an anti-apoptotic effect through down-regulation of miR-34a in a diabetic cardiomyopathy rat model. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN DIABETES ASSOC | - |
dc.title | Role of MicroRNA-34a in Anti-Apoptotic Effects of Granulocyte-Colony Stimulating Factor in Diabetic Cardiomyopathy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Yonggu | - |
dc.contributor.affiliatedAuthor | Shin, Jeong Hun | - |
dc.contributor.affiliatedAuthor | Lim, Young-Hyo | - |
dc.contributor.affiliatedAuthor | Kim, Hyuck | - |
dc.identifier.doi | 10.4093/dmj.2018.0211 | - |
dc.identifier.scopusid | 2-s2.0-85080952759 | - |
dc.identifier.wosid | 000517786100015 | - |
dc.identifier.bibliographicCitation | DIABETES & METABOLISM JOURNAL, v.44, no.1, pp.173 - 185 | - |
dc.relation.isPartOf | DIABETES & METABOLISM JOURNAL | - |
dc.citation.title | DIABETES & METABOLISM JOURNAL | - |
dc.citation.volume | 44 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 173 | - |
dc.citation.endPage | 185 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002563158 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.subject.keywordPlus | HIGH-FAT DIET | - |
dc.subject.keywordPlus | MYOCARDIAL-INFARCTION | - |
dc.subject.keywordPlus | HEART-FAILURE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTECTS | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | RAT | - |
dc.subject.keywordAuthor | Diabetic cardiomyopathies | - |
dc.subject.keywordAuthor | Granulocyte colony-stimulating factor | - |
dc.subject.keywordAuthor | MicroRNAs | - |
dc.identifier.url | https://www.e-dmj.org/journal/view.php?doi=10.4093/dmj.2018.0211 | - |
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