Overexpression of malic enzyme isoform 2 in Chlamydomonas reinhardtii PTS42 increases lipid production
DC Field | Value | Language |
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dc.contributor.author | Kim, Jongrae | - |
dc.contributor.author | Kwak, Ho Seok | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.contributor.author | Jin, EonSeon | - |
dc.date.accessioned | 2021-08-02T11:25:59Z | - |
dc.date.available | 2021-08-02T11:25:59Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2019-09 | - |
dc.identifier.issn | 2589-014X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/13204 | - |
dc.description.abstract | Genetic modification of microalgae has been performing to overcome the economic and technical difficulties associated with biodiesel production. In this study, to explore a new approach to exploiting a metabolic pathway for effective lipid production, we engineered Chlamydomonas reinhardtii PTS42 to overexpress malic enzyme isoform 2 (ME2) which catalyzes the conversion of malate to pyruvate. Overexpression of ME2 resulted in increase of mRNA level (around 2?3 fold) and increase of the pyruvate content (42.4% and 44.8% respectively) in transgenic M16 and M17, respectively. The fatty acid content and the lipid content of M17 were increased by 23.4 and 19.9% respectively compared to parental lines under the nitrogen deprivation condition. However, no significant changes in fatty acids composition was observed in transgenic lines. These results demonstrate that ME2 overexpression enhances the availability of pyruvate for fatty acid biosynthesis and lipid production in C. reinhardtii. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Ltd | - |
dc.title | Overexpression of malic enzyme isoform 2 in Chlamydomonas reinhardtii PTS42 increases lipid production | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jin, EonSeon | - |
dc.identifier.doi | 10.1016/j.biteb.2019.100239 | - |
dc.identifier.scopusid | 2-s2.0-85071785135 | - |
dc.identifier.bibliographicCitation | Bioresource Technology Reports, v.7 | - |
dc.relation.isPartOf | Bioresource Technology Reports | - |
dc.citation.title | Bioresource Technology Reports | - |
dc.citation.volume | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | lipid | - |
dc.subject.keywordPlus | malate dehydrogenase (decarboxylating) | - |
dc.subject.keywordPlus | messenger RNA | - |
dc.subject.keywordPlus | nitrogen | - |
dc.subject.keywordPlus | pyruvic acid | - |
dc.subject.keywordPlus | amino acid sequence | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | biomass production | - |
dc.subject.keywordPlus | catalyst | - |
dc.subject.keywordPlus | cell growth | - |
dc.subject.keywordPlus | Chlamydomonas reinhardtii | - |
dc.subject.keywordPlus | confocal microscopy | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | DNA integration | - |
dc.subject.keywordPlus | DNA sequence | - |
dc.subject.keywordPlus | fatty acid analysis | - |
dc.subject.keywordPlus | fatty acid synthesis | - |
dc.subject.keywordPlus | gene insertion | - |
dc.subject.keywordPlus | gene overexpression | - |
dc.subject.keywordPlus | genetic engineering | - |
dc.subject.keywordPlus | large scale production | - |
dc.subject.keywordPlus | lipid storage | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | real time polymerase chain reaction | - |
dc.subject.keywordPlus | Southern blotting | - |
dc.subject.keywordAuthor | Fatty acid | - |
dc.subject.keywordAuthor | Genetic modification | - |
dc.subject.keywordAuthor | Malic enzyme | - |
dc.subject.keywordAuthor | Microalgae | - |
dc.subject.keywordAuthor | Pyruvate | - |
dc.identifier.url | https://linkinghub.elsevier.com/retrieve/pii/S2589014X1930129X | - |
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