Analysis of MicroRNA Expression in Tears of Patients with Herpes Epithelial Keratitis: A Preliminary Study
DC Field | Value | Language |
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dc.contributor.author | Kim, Yu Jeong | - |
dc.contributor.author | Yeon, Yeji | - |
dc.contributor.author | Lee, Won June | - |
dc.contributor.author | Shin, Yong Un | - |
dc.contributor.author | Cho, Heeyoon | - |
dc.contributor.author | Lim, Han Woong | - |
dc.contributor.author | Kang, Min Ho | - |
dc.date.accessioned | 2022-07-06T04:10:19Z | - |
dc.date.available | 2022-07-06T04:10:19Z | - |
dc.date.created | 2022-06-03 | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/138787 | - |
dc.description.abstract | PURPOSE. Herpes epithelial keratitis (HEK) is the most common form of herpes simplex virus (HSV) eye involvement, and understanding the molecular mechanisms underlying HEK is important. We investigated the expression of microRNAs (miRNAs) in the tears of patients with HEK. METHODS. Tear samples from eight patients with HEK and seven age-matched controls were evaluated. Clinical ophthalmologic evaluation was performed, and an anterior segment photograph was obtained after fluorescence staining. Dendritic or geographic ulcer areas were measured using ImageJ software. The expression of 43 different miRNAs in tears was measured using real-time polymerase chain reaction and compared between patients with HEK and controls. Differences in miRNA expression between the dendritic and geographic ulcer groups and correlations involving miRNA expression and ulcer area were evaluated. RESULTS. Of the 43 miRNAs, 23 were upregulated in patients with HEK compared to normal controls. MiR-15b-5p, miR-16-5p, miR-20b-5p, miR-21-5p, miR-23b-3p, miR-25- 3p, miR-29a-3p, miR-30a-3p, miR-30d-5p, miR-92a-3p, miR-124-3p, miR-127-3p, miR- 132-3p, miR-142-3p, miR-145-5p, miR-146a-5p, miR-146b-5p, miR-155-5p, miR-182-5p, miR-183-5p, miR-221-3p, miR-223-3p, and miR-338-5p were significantly upregulated in patients with HEK. MiR-29a-3p exhibited significant differences between the dendritic and geographic ulcer groups. All 23 miRNAs with significant differences between patients with HEK and the control group were not significantly correlated with ulcer area. CONCLUSIONS. Twenty-three miRNAs were significantly upregulated in the tears of patients with HEK, and the expression of miRNAs may play important roles in herpes infection in relation to host immunity. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Association for Research in Vision and Ophthalmology Inc. | - |
dc.title | Analysis of MicroRNA Expression in Tears of Patients with Herpes Epithelial Keratitis: A Preliminary Study | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Won June | - |
dc.contributor.affiliatedAuthor | Shin, Yong Un | - |
dc.contributor.affiliatedAuthor | Lim, Han Woong | - |
dc.contributor.affiliatedAuthor | Kang, Min Ho | - |
dc.identifier.doi | 10.1167/iovs.63.4.21 | - |
dc.identifier.scopusid | 2-s2.0-85129779705 | - |
dc.identifier.bibliographicCitation | Investigative Ophthalmology and Visual Science, v.63, no.4, pp.1 - 8 | - |
dc.relation.isPartOf | Investigative Ophthalmology and Visual Science | - |
dc.citation.title | Investigative Ophthalmology and Visual Science | - |
dc.citation.volume | 63 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | hemoglobin A1c | - |
dc.subject.keywordPlus | interleukin 8 | - |
dc.subject.keywordPlus | long untranslated RNA | - |
dc.subject.keywordPlus | microRNA | - |
dc.subject.keywordPlus | microRNA 124 | - |
dc.subject.keywordPlus | microRNA 16 | - |
dc.subject.keywordPlus | microRNA 182 | - |
dc.subject.keywordPlus | microRNA 21 | - |
dc.subject.keywordPlus | prednisolone | - |
dc.subject.keywordPlus | RANTES | - |
dc.subject.keywordPlus | vasculotropin | - |
dc.subject.keywordPlus | microRNA | - |
dc.subject.keywordPlus | MIRN145 microRNA, human | - |
dc.subject.keywordPlus | MIRN338 microRNA, human | - |
dc.subject.keywordAuthor | biomarkers | - |
dc.subject.keywordAuthor | herpes epithelial keratitis | - |
dc.subject.keywordAuthor | microRNAs | - |
dc.subject.keywordAuthor | miRNAs | - |
dc.subject.keywordAuthor | tears | - |
dc.identifier.url | https://iovs.arvojournals.org/article.aspx?articleid=2778780 | - |
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