Engineering exosomes for pulmonary delivery of peptides and drugs to inflammatory lung cells by inhalation
DC Field | Value | Language |
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dc.contributor.author | Kim, GyeungYun | - |
dc.contributor.author | Lee, Youngki | - |
dc.contributor.author | Ha, Junkyu | - |
dc.contributor.author | Han, Sangrok | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2021-07-30T04:48:18Z | - |
dc.date.available | 2021-07-30T04:48:18Z | - |
dc.date.created | 2021-07-14 | - |
dc.date.issued | 2021-02 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/1393 | - |
dc.description.abstract | Exosomes have been investigated as delivery vesicles for various drugs. However, exosome-mediated peptide delivery into the lungs has not been studied. In this study, exosomes were engineered for the pulmonary delivery of RAGE-binding peptide (RBP), an anti-inflammatory peptide. To load the peptide into exosomes, RBP was linked to an exosome membrane integral protein, Lamp2b, to produce RBP-linked exosomes (RBP-exo). The anti-inflammatory effects of RBP-exo were confirmed by cytokine assays in lipopolysaccharide (LPS)-activated macrophage cells. To increase anti-inflammatory effects, curcumin was loaded into RBP-exo. Curcumin loaded RBP-exo (RBP-exo/Cur) had higher intracellular curcumin delivery efficiency than curcumin alone or curcumin loaded into unmodified exosomes (unmod-exo/Cur). This suggests that RBP on the surface of RBP-exo may interact with RAGE and increase the intracellular delivery efficiency of curcumin. In addition, RBP-exo/Cur had higher anti-inflammatory effects than curcumin alone, a mixture of RBP and curcumin, and unmod-exo/Cur in vitro. For in vivo evaluation, RBP-exo/Cur was administrated by intratracheal instillation into the lungs of an acute lung injury (ALI) model. The results showed that RBP-exo/Cur reduced pro-inflammatory cytokines more efficiently than curcumin alone, RBP-exo, and unmod-exo/Cur. Hematoxylin and eosin staining confirmed that the inflammation reaction was inhibited in the RBP-exo and RBP-exo/Cur groups. Immunostaining assays showed that RBP-exo was co-localized mostly with type I epithelial cells. In conclusion, RBP was successfully delivered with exosomes into the lungs by inhalation. A combination of RBP and curcumin using exosomes as carriers may be useful as ALI therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.title | Engineering exosomes for pulmonary delivery of peptides and drugs to inflammatory lung cells by inhalation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1016/j.jconrel.2020.12.053 | - |
dc.identifier.scopusid | 2-s2.0-85099149787 | - |
dc.identifier.wosid | 000626459100048 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.330, pp.684 - 695 | - |
dc.relation.isPartOf | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 330 | - |
dc.citation.startPage | 684 | - |
dc.citation.endPage | 695 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | Biological organs | - |
dc.subject.keywordPlus | Cell engineering | - |
dc.subject.keywordPlus | Efficiency | - |
dc.subject.keywordPlus | Peptides | - |
dc.subject.keywordPlus | Targeted drug delivery | - |
dc.subject.keywordPlus | binding protein | - |
dc.subject.keywordPlus | curcumin | - |
dc.subject.keywordPlus | eosin | - |
dc.subject.keywordPlus | hematoxylin | - |
dc.subject.keywordPlus | lysosome associated membrane protein 2 | - |
dc.subject.keywordPlus | rage binding peptide | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | curcumin | - |
dc.subject.keywordPlus | peptide | - |
dc.subject.keywordPlus | Activated macrophages | - |
dc.subject.keywordPlus | Anti-inflammatories | - |
dc.subject.keywordPlus | Anti-inflammatory effects | - |
dc.subject.keywordPlus | Intracellular delivery | - |
dc.subject.keywordPlus | Intratracheal instillation | - |
dc.subject.keywordPlus | Lipopolysaccharides | - |
dc.subject.keywordPlus | Pro-inflammatory cytokines | - |
dc.subject.keywordPlus | Pulmonary delivery | - |
dc.subject.keywordAuthor | Acute lung injury | - |
dc.subject.keywordAuthor | Curcumin | - |
dc.subject.keywordAuthor | Exosomes | - |
dc.subject.keywordAuthor | Pulmonary delivery | - |
dc.subject.keywordAuthor | RAGE-binding peptide | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S016836592030777X?via%3Dihub | - |
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