Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Gae-Baik | - |
dc.contributor.author | Lee, Jeong Min | - |
dc.contributor.author | Nguyen, Duc Long | - |
dc.contributor.author | Lee, Joonseok | - |
dc.contributor.author | Kim, Young-Pil | - |
dc.date.accessioned | 2022-07-06T10:17:02Z | - |
dc.date.available | 2022-07-06T10:17:02Z | - |
dc.date.created | 2022-03-07 | - |
dc.date.issued | 2022-02 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/139528 | - |
dc.description.abstract | Activity-based monitoring of cell-secreted proteases has gained significant interest due to the implication of these substances in diverse cellular functions. Here, we demonstrated a cell-based method of monitoring protease activity using fluorescent cell-permeable peptides. The activatable peptide consists of anionic (EEEE), cleavable, and cationic sequences (RRRR) that enable intracellular delivery by matrix metalloproteinase-2 (MMP2), which is secreted by living cancer cells. Compared to HT-29 cells (MMP2-negative), HT-1080 cells (MMP2-positive) showed a strong fluorescence response to the short fluorescent peptide via cell-secreted protease activation. Our approach is expected to find applications for the rapid visualization of protease activity in living cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Joonseok | - |
dc.contributor.affiliatedAuthor | Kim, Young-Pil | - |
dc.identifier.doi | 10.3390/ijms23031605 | - |
dc.identifier.scopusid | 2-s2.0-85123573433 | - |
dc.identifier.wosid | 000757405700001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.23, no.3, pp.1 - 12 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 23 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | MATRIX-METALLOPROTEINASE | - |
dc.subject.keywordPlus | PENETRATING PEPTIDES | - |
dc.subject.keywordPlus | GOLD NANOPARTICLE | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | MMP-9 | - |
dc.subject.keywordAuthor | activatable peptides | - |
dc.subject.keywordAuthor | fluorescent imaging | - |
dc.subject.keywordAuthor | proteases | - |
dc.subject.keywordAuthor | MMP2 | - |
dc.subject.keywordAuthor | cancer cells | - |
dc.identifier.url | https://www.mdpi.com/1422-0067/23/3/1605 | - |
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