Development of a New Approach for Low-Laser-Power Super-Resolution Fluorescence Imaging
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, Jinkyoung | - |
dc.contributor.author | Jeong, Uidon | - |
dc.contributor.author | Jeong, Dokyung | - |
dc.contributor.author | Go, Seokran | - |
dc.contributor.author | Kim, Doory | - |
dc.date.accessioned | 2022-07-06T10:46:05Z | - |
dc.date.available | 2022-07-06T10:46:05Z | - |
dc.date.created | 2021-12-08 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/139894 | - |
dc.description.abstract | The development of super-resolution fluorescence microscopy over the past decade has drastically improved the resolution of light microscopy to μ10 nm. Stochastic optical reconstruction microscopy (STORM) can be used to achieve subdiffraction-limit resolution by sequentially imaging and localizing individual fluorophores. In principle, the super-resolution of STORM can be obtained by high-accuracy localization of photoswitchable fluorophores, which require fast photoswitching and bright fluorescence intensity from a single emitter. It is known that the switching rate of photoswitchable fluorophores depends on the laser power-a high laser power being required for the enhancement of imaging resolution. However, high laser power is usually harmful to biological specimens and limits the imaging time because of its photobleaching effects and high phototoxicity. In this study, we attempted to overcome this problem by improving the STORM resolution at a lower laser power. Through the quantitative analysis of the photoswitching behavior of single fluorophores under different laser power conditions, we developed a new approach to achieve super-resolution fluorescence images at a laser power 10 times lower than had previously been reported. This approach is expected to play an increasingly significant role in super-resolution imaging of power-sensitive samples. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Development of a New Approach for Low-Laser-Power Super-Resolution Fluorescence Imaging | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Doory | - |
dc.identifier.doi | 10.1021/acs.analchem.1c01047 | - |
dc.identifier.scopusid | 2-s2.0-85119379868 | - |
dc.identifier.wosid | 000766200400012 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.94, no.2, pp.618 - 627 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 94 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 618 | - |
dc.citation.endPage | 627 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | Fluorescence imaging | - |
dc.subject.keywordPlus | Fluorescence microscopy | - |
dc.subject.keywordPlus | Fluorophores | - |
dc.subject.keywordPlus | Photobleaching | - |
dc.subject.keywordPlus | Stochastic systems | - |
dc.subject.keywordPlus | Storms | - |
dc.subject.keywordPlus | High laser power | - |
dc.subject.keywordPlus | Laser power | - |
dc.subject.keywordPlus | New approaches | - |
dc.subject.keywordPlus | Optical reconstruction | - |
dc.subject.keywordPlus | Photo-switchable | - |
dc.subject.keywordPlus | Photoswitching | - |
dc.subject.keywordPlus | Stochastics | - |
dc.subject.keywordPlus | Sub-diffraction limit | - |
dc.subject.keywordPlus | Superresolution | - |
dc.subject.keywordPlus | Optical resolving power | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acs.analchem.1c01047 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.