Down-regulation of TNF-alpha via macrophage-targeted RNAi system for the treatment of acute inflammatory sepsis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jieun | - |
dc.contributor.author | Son, Wooic | - |
dc.contributor.author | Hong, Juhyeong | - |
dc.contributor.author | Song, Yoonsung | - |
dc.contributor.author | Yang, Chul-Su | - |
dc.contributor.author | Kim, Yong-Hee | - |
dc.date.accessioned | 2022-07-06T16:01:49Z | - |
dc.date.available | 2022-07-06T16:01:49Z | - |
dc.date.issued | 2021-08 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.issn | 1873-4995 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/141387 | - |
dc.description.abstract | Sepsis is a systemic inflammatory response syndrome caused by bacterial infection. The sepsis therapy has involved prescription of adequate antibiotics, requiring several days to determine the proper type without reducing the inflammatory response. Thus, it is necessary to rapidly decrease fundamental inflammation, which can induce serious organ damage. In the inflammatory mechanism, tumor necrosis factor-alpha (TNF-alpha) produced by macrophages has an important role in infiltration of macrophages into infected sites and as a trigger for secretion of pro-inflammatory cytokines. However, commercialized TNF-alpha antibody medicines have limits such as fibrosis, cytokine storms, and high production costs. There is a growing need for anti-inflammatory sepsis treatment free from side effects. For this reason, TNF-alpha converting enzyme (TACE) could be an innovative target to break the positive feedback loop of inflammatory mediators (TNF-alpha) since it converts the inactive TNF-alpha membrane bound form to the activated soluble form in macrophages. A non-viral gene delivery system was developed in this study to deliver siRNA into inflammation-mediated macrophages without toxicity. The peptidebased gene carrier created by conjugating positively-charged nine arginine (9R) and the TKPR (Thr-Lys-Pro-Arg) sequence from the Fc region of Immunoglobulin G (IgG) specifically binds to the neuropilin-1 (NRP-1) receptor on the macrophage surface. Our results demonstrated that siTACE/TKPR-9R complexes were internalized in macrophages and successfully down-regulated TACE mRNA level. Finally, RNA interference with cell-targeted peptide carriers indicates a fundamental therapy for acute inflammatory sepsis free of off-target effects. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Down-regulation of TNF-alpha via macrophage-targeted RNAi system for the treatment of acute inflammatory sepsis | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1016/j.jconrel.2021.06.022 | - |
dc.identifier.scopusid | 2-s2.0-85111327718 | - |
dc.identifier.wosid | 000685045200005 | - |
dc.identifier.bibliographicCitation | Journal of Controlled Release, v.336, pp 344 - 353 | - |
dc.citation.title | Journal of Controlled Release | - |
dc.citation.volume | 336 | - |
dc.citation.startPage | 344 | - |
dc.citation.endPage | 353 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | TUMOR-NECROSIS-FACTOR | - |
dc.subject.keywordPlus | CONVERTING-ENZYME | - |
dc.subject.keywordPlus | PATHOPHYSIOLOGY | - |
dc.subject.keywordPlus | THERAPEUTICS | - |
dc.subject.keywordPlus | THERAPIES | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | LPS | - |
dc.subject.keywordAuthor | Targeted gene carrier | - |
dc.subject.keywordAuthor | Anti-inflammatory gene therapy | - |
dc.subject.keywordAuthor | RNA interference | - |
dc.subject.keywordAuthor | Combination therapy | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0168365921003096?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.