The Cysteine-Containing Cell-Penetrating Peptide AP Enables Efficient Macromolecule Delivery to T Cells and Controls Autoimmune Encephalomyelitis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Won-Ju | - |
dc.contributor.author | Kim, Gil-Ran | - |
dc.contributor.author | Cho, Hyun-Jung | - |
dc.contributor.author | Choi, Je-Min | - |
dc.date.accessioned | 2022-07-06T16:02:16Z | - |
dc.date.available | 2022-07-06T16:02:16Z | - |
dc.date.created | 2021-11-22 | - |
dc.date.issued | 2021-08 | - |
dc.identifier.issn | 1999-4923 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/141396 | - |
dc.description.abstract | T cells are key immune cells involved in the pathogenesis of several diseases, rendering them important therapeutic targets. Although drug delivery to T cells is the subject of continuous research, it remains challenging to deliver drugs to primary T cells. Here, we used a peptide-based drug delivery system, AP, which was previously developed as a transdermal delivery peptide, to modulate T cell function. We first identified that AP-conjugated enhanced green fluorescent protein (EGFP) was efficiently delivered to non-phagocytic human T cells. We also confirmed that a nine-amino acid sequence with one cysteine residue was the optimal sequence for protein delivery to T cells. Next, we identified the biodistribution of AP-dTomato protein in vivo after systemic administration, and transduced it to various tissues, such as the spleen, liver, intestines, and even to the brain across the blood-brain barrier. Next, to confirm AP-based T cell regulation, we synthesized the AP-conjugated cytoplasmic domain of CTLA-4, AP-ctCTLA-4 peptide. AP-ctCTLA-4 reduced IL-17A expression under Th17 differentiation conditions in vitro and ameliorated experimental autoimmune encephalomyelitis, with decreased numbers of pathogenic IL-17A(+)GM-CSF+ CD4 T cells. These results collectively suggest the AP peptide can be used for the successful intracellular regulation of T cell function, especially in the CNS. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | The Cysteine-Containing Cell-Penetrating Peptide AP Enables Efficient Macromolecule Delivery to T Cells and Controls Autoimmune Encephalomyelitis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Je-Min | - |
dc.identifier.doi | 10.3390/pharmaceutics13081134 | - |
dc.identifier.scopusid | 2-s2.0-85112652773 | - |
dc.identifier.wosid | 000690289800001 | - |
dc.identifier.bibliographicCitation | PHARMACEUTICS, v.13, no.8, pp.1 - 16 | - |
dc.relation.isPartOf | PHARMACEUTICS | - |
dc.citation.title | PHARMACEUTICS | - |
dc.citation.volume | 13 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 16 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | ATOPIC-DERMATITIS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | ENDOCYTOSIS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordAuthor | T cell | - |
dc.subject.keywordAuthor | immune regulation | - |
dc.subject.keywordAuthor | cell-penetrating peptide | - |
dc.subject.keywordAuthor | AP | - |
dc.subject.keywordAuthor | CTLA-4 | - |
dc.subject.keywordAuthor | multiple sclerosis | - |
dc.subject.keywordAuthor | experimental autoimmune encephalomyelitis EAE | - |
dc.subject.keywordAuthor | drug delivery system | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.